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Literature summary extracted from

  • Chung, J.H.; Suh, M.J.; Park, Y.I.; Tainer, J.A.; Han, Y.S.
    Repair activities of 8-oxoguanine DNA glycosylase from Archaeoglobus fulgidus, a hyperthermophilic archaeon (2001), Mutat. Res., 486, 99-111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.B5 overproduced in Escherichia coli as a maltose binding protein(MBP)-Afogg fusion protein Archaeoglobus fulgidus
4.2.99.18 overproduced in Escherichia coli as a maltose binding protein(MBP)-Afogg fusion protein Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.B5 NaCl catalytic activity is inhibited in buffer more than 100 mM NaCl Archaeoglobus fulgidus
4.2.99.18 NaCl catalytic activity is inhibited in buffer more than 100 mM NaCl Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.B5 22000
-
1 * 22000, SDS-PAGE Archaeoglobus fulgidus
3.2.2.B5 22639
-
1 * 22639, calculated from sequenec Archaeoglobus fulgidus
3.2.2.B5 23000
-
gel filtration Archaeoglobus fulgidus
3.2.2.B5 23124
-
1 * 23124, mass spectrophotometry Archaeoglobus fulgidus
4.2.99.18 22000
-
1 * 22000, SDS-PAGE Archaeoglobus fulgidus
4.2.99.18 22639
-
1 * 22639, calculated from sequenec Archaeoglobus fulgidus
4.2.99.18 23000
-
gel filtration Archaeoglobus fulgidus
4.2.99.18 23124
-
1 * 23124, mass spectrophotometry Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.B5 DNA containing 8-oxoguanine + H2O Archaeoglobus fulgidus the enzyme excises an oxidatively-damaged form of guanine. Bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities DNA with an abasic site + 8-oxoguanine
-
?
4.2.99.18 DNA with an abasic site Archaeoglobus fulgidus the enzyme excises an oxidatively-damaged form of guanine. Bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities DNA with 5'-phosphate terminus + DNA with 3'-alpha,beta-unsaturated aldehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.B5 Archaeoglobus fulgidus O29876
-
-
4.2.99.18 Archaeoglobus fulgidus O29876
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.B5 overproduced in Escherichia coli Archaeoglobus fulgidus
4.2.99.18 overproduced in Escherichia coli Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.B5 DNA containing 8-oxoguanine + H2O the enzyme excises an oxidatively-damaged form of guanine. Bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities Archaeoglobus fulgidus DNA with an abasic site + 8-oxoguanine
-
?
3.2.2.B5 DNA containing 8-oxoguanine + H2O bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities. The specificity of DNA glycosylase activity of is compared using 39-mer DNA duplexes containing an 8-oxoG residue opposite each of the four natural DNA bases (A, T, G and C). The enzyme efficiently cleaves oligomers containing 8-oxoG:C and 8-oxoG:G base pairs, less effective on oligomers containing 8-oxoG:T and 8-oxoG:A mispairs. The enzyme catalyzes a beta-elimination reaction at the apurinic site produced by excision of the 8-oxoguanine and thus generates a 5'-phosphate terminus and a 3'-alpha,beta-unsaturated aldehyde sugar terminus at the incision site Archaeoglobus fulgidus DNA with an abasic site + 8-oxoguanine
-
?
4.2.99.18 DNA with an abasic site the enzyme excises an oxidatively-damaged form of guanine. Bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities Archaeoglobus fulgidus DNA with 5'-phosphate terminus + DNA with 3'-alpha,beta-unsaturated aldehyde
-
?
4.2.99.18 DNA with an abasic site bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities. The specificity of DNA glycosylase activity of is compared using 39-mer DNA duplexes containing an 8-oxoG residue opposite each of the four natural DNA bases (A, T, G and C). The enzyme efficiently cleaves oligomers containing 8-oxoG:C and 8-oxoG:G base pairs, less effective on oligomers containing 8-oxoG:T and 8-oxoG:A mispairs. The enzyme catalyzes a beta-elimination reaction at the apurinic site produced by excision of the 8-oxoguanine and thus generates a 5'-phosphate terminus and a 3'-alpha,beta-unsaturated aldehyde sugar terminus at the incision site. Bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities Archaeoglobus fulgidus DNA with 5'-phosphate terminus + DNA with 3'-alpha,beta-unsaturated aldehyde
-
?

Subunits

EC Number Subunits Comment Organism
3.2.2.B5 monomer 1 * 22000, SDS-PAGE Archaeoglobus fulgidus
3.2.2.B5 monomer 1 * 22639, calculated from sequenec Archaeoglobus fulgidus
3.2.2.B5 monomer 1 * 23124, mass spectrophotometry Archaeoglobus fulgidus
4.2.99.18 monomer 1 * 22000, SDS-PAGE Archaeoglobus fulgidus
4.2.99.18 monomer 1 * 22639, calculated from sequenec Archaeoglobus fulgidus
4.2.99.18 monomer 1 * 23124, mass spectrophotometry Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
3.2.2.B5 8-oxoG DNA glycosylase
-
Archaeoglobus fulgidus
3.2.2.B5 Af0371
-
Archaeoglobus fulgidus
3.2.2.B5 Afogg enzyme bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities Archaeoglobus fulgidus
4.2.99.18 8-oxoG DNA glycosylase
-
Archaeoglobus fulgidus
4.2.99.18 Af0371
-
Archaeoglobus fulgidus
4.2.99.18 Afogg enzyme bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.B5 50
-
assay at Archaeoglobus fulgidus
3.2.2.B5 60
-
since the Tm-value of the DNA substrates (39-mer) of is 74°C, the activity above 60°C is not convincible. The actual optimum temperature of the enzyme might be higher than 60°C Archaeoglobus fulgidus
4.2.99.18 50
-
assay at Archaeoglobus fulgidus
4.2.99.18 60
-
since the Tm-value of the DNA substrates (39-mer) is 74°C, the activity above 60°C is not convincible. The actual optimum temperature of the enzyme might be higher than 60°C Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.B5 40 80 40°C: about 60% of maximal activity, 80°C: about 70% of maximal activity Archaeoglobus fulgidus
4.2.99.18 40 80 40°C: about 60% of maximal activity, 80°C: about 70% of maximal activity Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.B5 additional information
-
thermostability of the enzyme is the highest near 0.5 M NaCl Archaeoglobus fulgidus
3.2.2.B5 80
-
pH 7.5 (20 mM Tris–Cl buffer), denatured irreversibly above 80°C Archaeoglobus fulgidus
4.2.99.18 additional information
-
thermostability of the enzyme is the highest near 0.5 M NaCl Archaeoglobus fulgidus
4.2.99.18 80
-
pH 7.5 (20 mM Tris–Cl buffer), denatured irreversibly above 80°C Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.B5 8.5
-
-
Archaeoglobus fulgidus
4.2.99.18 8.5
-
-
Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.B5 7.5 10 pH 7.5: about 50% of maximal activity, pH 10.0: about 50% of maximal activity Archaeoglobus fulgidus
4.2.99.18 7.5 10 pH 7.5: about 50% of maximal activity, pH 10.0: about 50% of maximal activity Archaeoglobus fulgidus