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Literature summary extracted from

  • Villadangos, A.F.; Van Belle, K.; Wahni, K.; Dufe, V.T.; Freitas, S.; Nur, H.; De Galan, S.; Gil, J.A.; Collet, J.F.; Mateos, L.M.; Messens, J.
    Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms (2011), Mol. Microbiol., 82, 998-1014.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.20.4.4 expression in Escherichia coli Corynebacterium glutamicum
2.8.4.2 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.20.4.4 seleno-methionine derivative, to 2.2 A resolution Corynebacterium glutamicum
2.8.4.2 isoform ArsC2, hanging drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5 Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.20.4.4 additional information in mutant lacking N-terminal 78 amino acids, the kinetic parameters are greatly reduced Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.4.4 0.032
-
 arsenate wild-type, pH 8.0, 37°C Corynebacterium glutamicum
1.20.4.4 0.11
-
 arsenate N-terminally truncated mutant, pH 8.0, 37°C Corynebacterium glutamicum
2.8.4.2 0.082
-
 arsenate isoform ArsC2, pH and temperature not specified in the publication Corynebacterium glutamicum
2.8.4.2 0.142
-
 arsenate isoform ArsC1, pH and temperature not specified in the publication Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.4.4 25360
-
 2 * 25360, SDS-PAGE Corynebacterium glutamicum
1.20.4.4 60000
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.2 arsenate + mycothiol Corynebacterium glutamicum
-
 arseno-mycothiol + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.20.4.4 Corynebacterium glutamicum P0DKS5 enzyme additionally shows low phosphates activity
-
1.20.4.4 Corynebacterium glutamicum DSM 20300 P0DKS5 enzyme additionally shows low phosphates activity
-
2.8.4.2 Corynebacterium glutamicum P0DKS7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.2
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.4.4 arsenate + thioredoxin
-
 Corynebacterium glutamicum arsenite + thioredoxin disulfide + H2O
-
 ?
1.20.4.4 arsenate + thioredoxin
-
 Corynebacterium glutamicum DSM 20300 arsenite + thioredoxin disulfide + H2O
-
 ?
2.8.4.2 arsenate + mycothiol
-
 Corynebacterium glutamicum arseno-mycothiol + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.4.4 dimer 2 * 25360, SDS-PAGE Corynebacterium glutamicum
1.20.4.4 More isoform ArsC1' is unique in bearing an N-terminal three-helical bundle that interacts with the active site of the other chain in the dimeric interface Corynebacterium glutamicum
2.8.4.2 monomer
-
 Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
1.20.4.4 ArsC1'
-
 Corynebacterium glutamicum
2.8.4.2 ArsC1 isoform Corynebacterium glutamicum
2.8.4.2 ArsC2 isoform Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.20.4.4 0.09
-
 arsenate wild-type, pH 8.0, 37°C Corynebacterium glutamicum
1.20.4.4 0.5
-
 arsenate N-terminally truncated mutant, pH 8.0, 37°C Corynebacterium glutamicum
2.8.4.2 0.28
-
 arsenate isoform ArsC2, pH and temperature not specified in the publication Corynebacterium glutamicum
2.8.4.2 0.53
-
 arsenate isoform ArsC1, pH and temperature not specified in the publication Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.4.4 additional information no cofactor: mycoredoxin Corynebacterium glutamicum
1.20.4.4 thioredoxin
-
 Corynebacterium glutamicum

Expression

EC Number Organism Comment Expression
1.20.4.4 Corynebacterium glutamicum isooform ArsC1' is constitutively expressed at low levels using its own promoter site. It reduces arsenate to arsenite that can then induce the expression of isoforms ArsC1 and ArsC2 additional information

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.20.4.4 0.045
-
 arsenate N-terminally truncated mutant, pH 8.0, 37°C Corynebacterium glutamicum
1.20.4.4 2.9
-
 arsenate wild-type, pH 8.0, 37°C Corynebacterium glutamicum
2.8.4.2 3.4
-
 arsenate isoform ArsC2, pH and temperature not specified in the publication Corynebacterium glutamicum
2.8.4.2 3.8
-
 arsenate isoform ArsC1, pH and temperature not specified in the publication Corynebacterium glutamicum