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Literature summary extracted from

  • Matsubara, K.; Yokooji, Y.; Atomi, H.; Imanaka, T.
    Biochemical and genetic characterization of the three metabolic routes in Thermococcus kodakarensis linking glyceraldehyde 3-phosphate and 3-phosphoglycerate (2011), Mol. Microbiol., 81, 1300-1312.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.9 D-glucose 1-phosphate
-
 Thermococcus kodakarensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.9 expressed in Escherichia coli BL21(DE3) CodonPlus RIL cells Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.9 D-glyceraldehyde 3-phosphate substrate inhibition Thermococcus kodakarensis
1.2.1.9 L-Glyceraldehyde 3-phosphate non-competitive inhibition Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.9 55486
-
 4 * 55486, calculated from amino acid sequence Thermococcus kodakarensis
1.2.1.9 230000
-
 gel filtration Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.9 DL-glyceraldehyde 3-phosphate + NADP+ + H2O Thermococcus kodakarensis
-
 3-phospho-DL-glycerate + NADPH + H+
-
 ?
1.2.1.9 additional information Thermococcus kodakarensis the enzyme does not react with formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, valeraldehyde, caproaldehyde, betaine aldehyde, glycolaldehyde, succinic semialdehyde or glyceraldehyde ?
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+ Thermococcus kodakarensis
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.9 Thermococcus kodakarensis
-
-
-
1.2.1.13 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.9 heat treatment (85°C, 30 min), anion exchange chromatography and gel filtration Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.9 DL-glyceraldehyde 3-phosphate + NADP+ + H2O
-
 Thermococcus kodakarensis 3-phospho-DL-glycerate + NADPH + H+
-
 ?
1.2.1.9 additional information the enzyme does not react with formaldehyde, acetaldehyde, propionaldehyde, butylaldehyde, valeraldehyde, caproaldehyde, betaine aldehyde, glycolaldehyde, succinic semialdehyde or glyceraldehyde Thermococcus kodakarensis ?
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Thermococcus kodakarensis 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.9 homotetramer 4 * 55486, calculated from amino acid sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.9 GAPN
-
 Thermococcus kodakarensis
1.2.1.9 non-phosphorylating GAP dehydrogenase
-
 Thermococcus kodakarensis
1.2.1.13 GAPDH
-
 Thermococcus kodakarensis
1.2.1.13 phosphorylating GAP dehydrogenase
-
 Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.9 NAD+ enzyme activity is observed with both NAD+ and NADP+, but NADP+ is the preferred cofactor Thermococcus kodakarensis
1.2.1.9 NADP+ enzyme activity is observed with both NAD+ and NADP+, but NADP+ is the preferred cofactor Thermococcus kodakarensis
1.2.1.13 NADP+
-
 Thermococcus kodakarensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.9 36.5
-
 D-glyceraldehyde 3-phosphate in 100 mM MES/KOH (pH 7.0), at 70°C Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
1.2.1.9 malfunction DELTAgapN cells do not grow under glycolytic conditions Thermococcus kodakarensis
1.2.1.9 metabolism the enzyme plays a key role in providing NADPH under glycolytic conditions Thermococcus kodakarensis
1.2.1.13 malfunction a deletion mutant strain does not exhibit any growth under gluconeogenic conditions Thermococcus kodakarensis
1.2.1.13 metabolism the enzyme plays a major role only in gluconeogenesis Thermococcus kodakarensis