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Literature summary extracted from

  • Lo, H.F.; Chen, Y.J.
    Gene cloning and biochemical characterization of a NAD(P)+ -dependent aldehyde dehydrogenase from Bacillus licheniformis (2010), Mol. Biotechnol., 46, 157-167.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.5 2-mercaptoethanol 246% activity at 2 mM Bacillus licheniformis
1.2.1.5 dithiothreitol 276% activity at 2 mM Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.5 expressed in Escherichia coli M15(pREP4) cells Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.5 1,2-Cyclohexanedione 33% residual activity at 2.5 mM Bacillus licheniformis
1.2.1.5 2,4-Dinitro-1-fluorobenzene 11% residual activity at 2.5 mM Bacillus licheniformis
1.2.1.5 Co2+ 89% residual activity at 1 mM Bacillus licheniformis
1.2.1.5 Cu2+ 20% residual activity at 1 mM Bacillus licheniformis
1.2.1.5 diethyl dicarbonate 2% residual activity at 2.5 mM Bacillus licheniformis
1.2.1.5 ethyl acetimidate 71% residual activity at 250 mM Bacillus licheniformis
1.2.1.5 Fe2+ 88% residual activity at 1 mM Bacillus licheniformis
1.2.1.5 Hg2+ complete inhibition at 0.25 mM Bacillus licheniformis
1.2.1.5 Mg2+ 89% residual activity at 1 mM Bacillus licheniformis
1.2.1.5 Mn2+ 89% residual activity at 1 mM Bacillus licheniformis
1.2.1.5 additional information not inhibited by EDTA Bacillus licheniformis
1.2.1.5 N-Acetylimidazole 75% residual activity at 2.5 mM Bacillus licheniformis
1.2.1.5 N-bromosuccinimide 66% residual activity at 0.5 mM Bacillus licheniformis
1.2.1.5 phenylmethylsulfonyl fluoride 73% residual activity at 2.5 mM Bacillus licheniformis
1.2.1.5 Woodward's reagent K 71% residual activity at 50 mM Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.5 0.12
-
 Valeraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 0.34
-
 Valeraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 0.46
-
 propionaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 0.65
-
 propionaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 2.95
-
 Butyraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 4.93
-
 Butyraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 9.87
-
 Isovaleraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 10.78
-
 Isovaleraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 18.89
-
 acetaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 393.4
-
 acetaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.5 additional information the enzyme does not require metal ions for its enzymatic reaction Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 52682
-
 4 * 52682, calculated from amino acid sequence Bacillus licheniformis
1.2.1.5 53000
-
 4 * 53000, calculated from amino acid sequence Bacillus licheniformis
1.2.1.5 220000
-
 gel filtration Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.5 Bacillus licheniformis
-
-
-
1.2.1.5 Bacillus licheniformis ATCC 14580
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.5 Ni2+-NTA resin column chromatography Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.5 acetaldehyde + NAD+
-
 Bacillus licheniformis acetate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NADP+
-
 Bacillus licheniformis acetate + NADPH + H+
-
 ?
1.2.1.5 butyraldehyde + NAD+
-
 Bacillus licheniformis butyrate + NADH + H+
-
 ?
1.2.1.5 butyraldehyde + NADP+
-
 Bacillus licheniformis butyrate + NADPH + H+
-
 ?
1.2.1.5 isovaleraldehyde + NAD+
-
 Bacillus licheniformis isovalerate + NADH + H+
-
 ?
1.2.1.5 isovaleraldehyde + NADP+
-
 Bacillus licheniformis isovalerate + NADPH + H+
-
 ?
1.2.1.5 isovaleraldehyde + NADP+
-
 Bacillus licheniformis ATCC 14580 isovalerate + NADPH + H+
-
 ?
1.2.1.5 additional information no activity with formaldehyde and benzaldehyde Bacillus licheniformis ?
-
 ?
1.2.1.5 additional information no activity with formaldehyde and benzaldehyde Bacillus licheniformis ATCC 14580 ?
-
 ?
1.2.1.5 n-propanal + NADP+
-
 Bacillus licheniformis propionate + NADPH + H+
-
 ?
1.2.1.5 propionaldehyde + NAD+ + H2O best substrate combination Bacillus licheniformis propionate + NADH + H+
-
 ?
1.2.1.5 propionaldehyde + NAD+ + H2O best substrate combination Bacillus licheniformis ATCC 14580 propionate + NADH + H+
-
 ?
1.2.1.5 propionaldehyde + NADP+ + H2O
-
 Bacillus licheniformis propionate + NADPH + H+
-
 ?
1.2.1.5 propionaldehyde + NADP+ + H2O
-
 Bacillus licheniformis ATCC 14580 propionate + NADPH + H+
-
 ?
1.2.1.5 valeraldehyde + NAD+
-
 Bacillus licheniformis valerate + NADH + H+
-
 ?
1.2.1.5 valeraldehyde + NADP+
-
 Bacillus licheniformis valerate + NADPH + H+
-
 ?
1.2.1.5 valeraldehyde + NADP+
-
 Bacillus licheniformis ATCC 14580 valerate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.5 homotetramer 4 * 52682, calculated from amino acid sequence Bacillus licheniformis
1.2.1.5 homotetramer 4 * 53000, calculated from amino acid sequence Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.5 aldehyde: NAD(P)+ oxidoreductase
-
 Bacillus licheniformis
1.2.1.5 ALDH
-
 Bacillus licheniformis
1.2.1.5 NAD(P)+-dependent aldehyde dehydrogenase
-
 Bacillus licheniformis
1.2.1.5 ybcD
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.5 37
-
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.5 20 40 the enzyme appears to be stable at 20°C, but becomes very unstable at a temperature above 37°C, most likely due to thermal denaturation Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.5 0.91
-
 Isovaleraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 1.11
-
 acetaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 3.58
-
 Isovaleraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 4.46
-
 acetaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 11.5
-
 Butyraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 13.36
-
 propionaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 19.41
-
 Valeraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 30.99
-
 Valeraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 32.8
-
 Butyraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 49.38
-
 propionaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.5 7
-
-
 Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.5 4 6 pH 6.0 attenuates more than 39% of enzymatic activity. At pH 5.0, 3% of the activity remains. The enzymatic activity is completely abolished at pH values of or below 4.0 Bacillus licheniformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.5 NAD+ preferred cofactor Bacillus licheniformis
1.2.1.5 NADP+
-
 Bacillus licheniformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.5 0.003
-
 acetaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 0.09
-
 Isovaleraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 0.24
-
 acetaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 0.36
-
 Isovaleraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 2.39
-
 Butyraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 11.89
-
 Butyraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 20.67
-
 propionaldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 56.66
-
 Valeraldehyde with NADP+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 107.5
-
 propionaldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis
1.2.1.5 257
-
 Valeraldehyde with NAD+ as cosubstrate, in 50 mM potassium phosphate buffer (pH 7.0), 1 mM dithiothreitol, at 37°C Bacillus licheniformis