EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.206 | synthesis | transgenic hairy root lines expressing both tropinone reductase I and hyoscyamine-6beta-hydroxylase produce significantly higher levels of tropinone alkaloids compared with the control and single gene transformed lines, reaching up to 4.293 mg/g tropinone alkaloids. In addition, the content of anisodine is also greatly improved. The average content of anisodine in double transformed lines is 0.984 mg/g dry weight, about 18fold of control lines | Anisodus acutangulus |
1.14.11.11 | pharmacology | tropane alkaloids including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, improvement of production by metabolic engineering introduction of genes encoding the branch-controlling enzyme tropinone reductase I and the downstream rate-limiting enzyme hyoscyamine-6beta-hydroxylase | Anisodus acutangulus |
1.14.20.13 | pharmacology | tropane alkaloids including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, improvement of production by metabolic engineering introduction of genes encoding the branch-controlling enzyme tropinone reductase I and the downstream rate-limiting enzyme hyoscyamine-6beta-hydroxylase | Anisodus acutangulus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.11.11 | overexpression of the enzyme in hairy roots of transgenic Anisodus acutangulus using Agrobacterium tumefaciens strain C58C1 transfection method, coexpression with tropinone reductase I | Anisodus acutangulus |
1.14.20.13 | overexpression of the enzyme in hairy roots of transgenic Anisodus acutangulus using Agrobacterium tumefaciens strain C58C1 transfection method, coexpression with tropinone reductase I | Anisodus acutangulus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.11.11 | additional information | enhanced production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropan alkaloid contents in transgenic lines, overview | Anisodus acutangulus |
1.14.20.13 | additional information | enhancied production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase Agrobacterium-mediated gene transfer technology, quantification of tropanalkaloid contents in transgenic lines, overview | Anisodus acutangulus |
1.14.20.13 | additional information | enhancied production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by overexpressing tropinone reductase I and hyoscyamine-6beta-hydroxylase, quantification of tropan alkaloid contents in transgenic lines, overview | Anisodus acutangulus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.11 | L-hyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
(6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
1.14.20.13 | (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
scopolamine + succinate + CO2 | - |
? | |
1.14.20.13 | (6S)-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Anisodus acutangulus | - |
scopolamine + succinate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.206 | Anisodus acutangulus | B2L2W8 | isoform tropinone reductase I | - |
1.14.11.11 | Anisodus acutangulus | A2IBI0 | - |
- |
1.14.20.13 | Anisodus acutangulus | - |
- |
- |
1.14.20.13 | Anisodus acutangulus | A2IBI0 | cf. EC 1.14.11.11 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.11.11 | hairy root | - |
Anisodus acutangulus | - |
1.14.20.13 | hairy root | - |
Anisodus acutangulus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.11 | L-hyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | (6S)-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
1.14.11.11 | additional information | the enzyme also catalyzes the reaction of EC 1.14.11.14 | Anisodus acutangulus | ? | - |
? | |
1.14.20.13 | (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | scopolamine + succinate + CO2 | - |
? | |
1.14.20.13 | (6S)-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Anisodus acutangulus | scopolamine + succinate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.11.11 | H6H | - |
Anisodus acutangulus |
1.14.11.11 | hyoscyamine-6beta-hydroxylase | - |
Anisodus acutangulus |
1.14.20.13 | H&H | - |
Anisodus acutangulus |
1.14.20.13 | H6H | - |
Anisodus acutangulus |
1.14.20.13 | hyoscyamine-6beta-hydroxylase | - |
Anisodus acutangulus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.11.11 | metabolism | the enzyme is involved in biosynthesis pathway of tropane alkaloids catalyzing two oxidation steps to form scopolamine from hyoscyamine, overview, cf. EC 1.14.11.14 | Anisodus acutangulus |
1.14.20.13 | metabolism | the enzyme is involved in biosynthesis pathway of tropane alkaloids catalyzing two oxidation steps to form scopolamine from hyoscyamine, overview, cf. EC 1.14.11.11 | Anisodus acutangulus |