Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Evitt, A.S.; Cox, R.J.
    Synthesis and evaluation of conformationally restricted inhibitors of aspartate semialdehyde dehydrogenase (2011), Mol. Biosyst., 7, 1564-1575.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expressed in Escherichia coli Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.11 cis-5-phosphonic acid pipecolic acid
-
 Salmonella enterica
1.2.1.11 pipecolic acid-5-(R)-phosphate hydrochloric acid
-
 Salmonella enterica
1.2.1.11 pipecolic acid-5-(S)-phosphate hydrochloric acid
-
 Salmonella enterica
1.2.1.11 trans-5-phosphonic acid pipecolic acid
-
 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.11 0.032
-
 phosphate in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica
1.2.1.11 0.16
-
 L-4-aspartyl phosphate in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica
1.2.1.11 0.17
-
 L-aspartate 4-semialdehyde in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.11 39000
-
 2 * 39000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+ Salmonella enterica
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Salmonella enterica
-
 L-4-aspartyl phosphate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11
-
 Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+
-
 Salmonella enterica L-aspartate 4-semialdehyde + phosphate + NADP+
-
 r
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Salmonella enterica L-4-aspartyl phosphate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.11 homodimer 2 * 39000, SDS-PAGE Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Salmonella enterica
1.2.1.11 aspartate semialdehyde dehydrogenase
-
 Salmonella enterica

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Salmonella enterica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.11 236
-
 L-aspartate 4-semialdehyde in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica
1.2.1.11 265
-
 phosphate in 0.2 M bicine buffer, pH 8.6, at 25°C Salmonella enterica