BRENDA - Enzyme Database show

Synthesis and evaluation of conformationally restricted inhibitors of aspartate semialdehyde dehydrogenase

Evitt, A.S.; Cox, R.J.; Mol. Biosyst. 7, 1564-1575 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.11
expressed in Escherichia coli
Salmonella enterica
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
cis-5-phosphonic acid pipecolic acid
-
Salmonella enterica
1.2.1.11
pipecolic acid-5-(R)-phosphate hydrochloric acid
-
Salmonella enterica
1.2.1.11
pipecolic acid-5-(S)-phosphate hydrochloric acid
-
Salmonella enterica
1.2.1.11
trans-5-phosphonic acid pipecolic acid
-
Salmonella enterica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.032
-
phosphate
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
1.2.1.11
0.16
-
L-4-aspartyl phosphate
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
1.2.1.11
0.17
-
L-aspartate 4-semialdehyde
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
39000
-
2 * 39000, SDS-PAGE
Salmonella enterica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH + H+
Salmonella enterica
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Salmonella enterica
-
L-4-aspartyl phosphate + NADPH + H+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.11
Salmonella enterica
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.11
-
Salmonella enterica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH + H+
-
725944
Salmonella enterica
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
725944
Salmonella enterica
L-4-aspartyl phosphate + NADPH + H+
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
2 * 39000, SDS-PAGE
Salmonella enterica
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Salmonella enterica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
expressed in Escherichia coli
Salmonella enterica
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.11
NADP+
-
Salmonella enterica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.11
cis-5-phosphonic acid pipecolic acid
-
Salmonella enterica
1.2.1.11
pipecolic acid-5-(R)-phosphate hydrochloric acid
-
Salmonella enterica
1.2.1.11
pipecolic acid-5-(S)-phosphate hydrochloric acid
-
Salmonella enterica
1.2.1.11
trans-5-phosphonic acid pipecolic acid
-
Salmonella enterica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.11
0.032
-
phosphate
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
1.2.1.11
0.16
-
L-4-aspartyl phosphate
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
1.2.1.11
0.17
-
L-aspartate 4-semialdehyde
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.11
39000
-
2 * 39000, SDS-PAGE
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH + H+
Salmonella enterica
-
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
Salmonella enterica
-
L-4-aspartyl phosphate + NADPH + H+
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.11
-
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.11
L-4-aspartyl phosphate + NADPH + H+
-
725944
Salmonella enterica
L-aspartate 4-semialdehyde + phosphate + NADP+
-
-
-
r
1.2.1.11
L-aspartate 4-semialdehyde + phosphate + NADP+
-
725944
Salmonella enterica
L-4-aspartyl phosphate + NADPH + H+
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.11
homodimer
2 * 39000, SDS-PAGE
Salmonella enterica
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2.1.11
236
-
L-aspartate 4-semialdehyde
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
1.2.1.11
265
-
phosphate
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.2.1.11
236
-
L-aspartate 4-semialdehyde
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica
1.2.1.11
265
-
phosphate
in 0.2 M bicine buffer, pH 8.6, at 25°C
Salmonella enterica