Literature summary extracted from
Xu, F.; Li, L.; Zhang, W.; Cheng, H.; Sun, N.; Cheng, S.; Wang, Y.
Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba (2012), Mol. Biol. Rep., 39, 2285-2296.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.20.6 |
gene GbFLS, DNA and amino acid sequence determination and analysis, promoter region sequence analysis and putative cis-acting regulatory elements, overview, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
Ginkgo biloba |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.20.6 |
Fe2+ |
dependent on |
Ginkgo biloba |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.14.20.6 |
(2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 |
Ginkgo biloba |
- |
kaempferol + succinate + CO2 + H2O |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.20.6 |
Ginkgo biloba |
D0UZK7 |
gene GbFLS |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.20.6 |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography |
Ginkgo biloba |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.20.6 |
(2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 |
- |
Ginkgo biloba |
kaempferol + succinate + CO2 + H2O |
- |
? |
|
1.14.20.6 |
naringenin + 2-oxoglutarate + O2 |
- |
Ginkgo biloba |
kaempferol + succinate + CO2 + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.20.6 |
FLS |
- |
Ginkgo biloba |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.20.6 |
30 |
- |
assay at |
Ginkgo biloba |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.20.6 |
7.5 |
- |
assay at |
Ginkgo biloba |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
1.14.20.6 |
Ginkgo biloba |
GbFLS expression is induced by abiotic stresses: UV-B irradiation, abscisic acid, cold, sucrose, salicylic acid, and ethephon |
up |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.14.20.6 |
evolution |
the enzyme belongs to the 2-oxoglutarate iron-dependent oxygenase family |
Ginkgo biloba |