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Literature summary extracted from

  • Xu, F.; Li, L.; Zhang, W.; Cheng, H.; Sun, N.; Cheng, S.; Wang, Y.
    Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba (2012), Mol. Biol. Rep., 39, 2285-2296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.6 gene GbFLS, DNA and amino acid sequence determination and analysis, promoter region sequence analysis and putative cis-acting regulatory elements, overview, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Ginkgo biloba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.6 Fe2+ dependent on Ginkgo biloba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.6 (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 Ginkgo biloba
-
kaempferol + succinate + CO2 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.6 Ginkgo biloba D0UZK7 gene GbFLS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.6 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography Ginkgo biloba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.6 (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2
-
Ginkgo biloba kaempferol + succinate + CO2 + H2O
-
?
1.14.20.6 naringenin + 2-oxoglutarate + O2
-
Ginkgo biloba kaempferol + succinate + CO2 + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.6 FLS
-
Ginkgo biloba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.20.6 30
-
assay at Ginkgo biloba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.6 7.5
-
assay at Ginkgo biloba

Expression

EC Number Organism Comment Expression
1.14.20.6 Ginkgo biloba GbFLS expression is induced by abiotic stresses: UV-B irradiation, abscisic acid, cold, sucrose, salicylic acid, and ethephon up

General Information

EC Number General Information Comment Organism
1.14.20.6 evolution the enzyme belongs to the 2-oxoglutarate iron-dependent oxygenase family Ginkgo biloba