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Literature summary extracted from

  • Biyani, N.; Madhubala, R.
    Leishmania donovani encodes a functional enzyme involved in vitamin C biosynthesis: arabino-1,4-lactone oxidase (2011), Mol. Biochem. Parasitol., 180, 76-85.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.37 gene LdALO, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of maltose binding protein-tagged LdALO in Escherichia coli strain BL21 (DE3), overexpressioin Leishmania donovani Leishmania donovani

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.37 additional information overexpression of LdALO in Leishmannia donovani results in better ability of survival of the parasite within the host in comparison to the vector transfectants Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.37 HgCl2 68% inhibition at 0.05 mM, complete inhibition at 1 mM Leishmania donovani
1.1.3.37 N-ethylmaleimide complete inhibition at 0.05 mM Leishmania donovani
1.1.3.37 ZnCl2 17% inhibition at 0.05 mM, 75% inhibition at 1 mM Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.37 additional information
-
 additional information Michaelis-Menten kinetics, overview Leishmania donovani
1.1.3.37 0.0393
-
 D-arabinono-1,4-lactone pH 7.5, 22°C Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.37 glycosome
-
 Leishmania donovani 20015
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.37 57000
-
 x * 57000, SDS-PAGE Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.37 D-arabinono-1,4-lactone + O2 Leishmania donovani
-
 dehydro-D-arabinono-1,4-lactone + H2O2
-
 ?
1.1.3.37 D-arabinono-1,4-lactone + O2 Leishmania donovani AG83
-
 dehydro-D-arabinono-1,4-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.37 Leishmania donovani C8CCV9
-
-
1.1.3.37 Leishmania donovani AG83 C8CCV9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.37 recombinant maltose binding protein-tagged LdALO from Escherichia coli strain BL21 (DE3) by amylose affinity chromatography, Factor Xa cleavage of maltose binding protein-tag Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.37 D-arabinono-1,4-lactone + O2
-
 Leishmania donovani dehydro-D-arabinono-1,4-lactone + H2O2
-
 ?
1.1.3.37 D-arabinono-1,4-lactone + O2
-
 Leishmania donovani AG83 dehydro-D-arabinono-1,4-lactone + H2O2
-
 ?
1.1.3.37 additional information no activity with L-gulono-1,4-lactone Leishmania donovani ?
-
 ?
1.1.3.37 additional information no activity with L-gulono-1,4-lactone Leishmania donovani AG83 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.37 ? x * 57000, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
1.1.3.37 ALO
-
 Leishmania donovani
1.1.3.37 arabino-1,4-lactone oxidase
-
 Leishmania donovani
1.1.3.37 arabinonolactone oxidase
-
 Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.37 22
-
 assay at room temperature Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.37 10.8
-
 D-arabinono-1,4-lactone pH 7.5, 22°C Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.37 7.5
-
 assay at Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.37 FAD essentially required Leishmania donovani

General Information

EC Number General Information Comment Organism
1.1.3.37 evolution the enzyme belongs to the family of flavoprotein aldonolactone oxidase/dehydrogenases Leishmania donovani
1.1.3.37 metabolism the enzyme catalyzes the last step in the biosynthesis of L-ascorbic acid involving the conversion of an aldonolactone substrate to ascorbate (or analogues) Leishmania donovani
1.1.3.37 additional information cysteine residues are required for enzyme activity Leishmania donovani
1.1.3.37 physiological function D-arabinono-1,4-lactone oxidase is required for synthesizing ascorbate in Leishmania Leishmania donovani