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Literature summary extracted from

  • Sygmund, C.; Klausberger, M.; Felice, A.K.; Ludwig, R.
    Reduction of quinones and phenoxy radicals by extracellular glucose dehydrogenase from Glomerella cingulata suggests a role in plant pathogenicity (2011), Microbiology, 157, 3203-3212.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 DNA and amino acid sequence determination and analysis, phylogenetic analysis Colletotrichum gloeosporioides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.9 extracellular
-
 Colletotrichum gloeosporioides
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 68000
-
 1 * 68000, deglycosylated enzyme, SDS-PAGE Colletotrichum gloeosporioides
1.1.5.9 95000 135000 glycoprotein, native PAGE Colletotrichum gloeosporioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.9 D-glucose + a quinone Colletotrichum gloeosporioides
-
 D-glucono-1,5-lactone + a quinol
-
 ?
1.1.5.9 D-glucose + a quinone Colletotrichum gloeosporioides DSM 62728
-
 D-glucono-1,5-lactone + a quinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Colletotrichum gloeosporioides
-
 anamorph Colletotrichum gloeosporoides
-
1.1.5.9 Colletotrichum gloeosporioides DSM 62728
-
 anamorph Colletotrichum gloeosporoides
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.5.9 glycoprotein four potential N-glycosylation sites at N55, N233, N255 and N339 Colletotrichum gloeosporioides

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration Colletotrichum gloeosporioides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.9 565
-
 purified enzyme with ferrocenium hexafluorophosphate, pH 5.5, 30°C Colletotrichum gloeosporioides
1.1.5.9 840
-
 purified enzyme with 2,6-dichlorophenol indophenol , pH 5.5, 30°C Colletotrichum gloeosporioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + 1,4-benzoquinone
-
 Colletotrichum gloeosporioides D-glucono-1,5-lactone + 1,4-benzoquinol
-
 ?
1.1.5.9 D-glucose + 1,4-benzoquinone
-
 Colletotrichum gloeosporioides DSM 62728 D-glucono-1,5-lactone + 1,4-benzoquinol
-
 ?
1.1.5.9 D-glucose + a quinone
-
 Colletotrichum gloeosporioides D-glucono-1,5-lactone + a quinol
-
 ?
1.1.5.9 D-glucose + a quinone
-
 Colletotrichum gloeosporioides DSM 62728 D-glucono-1,5-lactone + a quinol
-
 ?
1.1.5.9 D-glucose + ferricenium hexafluorophosphate
-
 Colletotrichum gloeosporioides D-glucono-1,5-lactone + ferrocenium hexafluorophosphate
-
 ?
1.1.5.9 D-glucose + ferricenium hexafluorophosphate
-
 Colletotrichum gloeosporioides DSM 62728 D-glucono-1,5-lactone + ferrocenium hexafluorophosphate
-
 ?
1.1.5.9 D-glucose + ferricyanide
-
 Colletotrichum gloeosporioides D-glucono-1,5-lactone + ferrocyanide
-
 ?
1.1.5.9 D-glucose + ferricyanide
-
 Colletotrichum gloeosporioides DSM 62728 D-glucono-1,5-lactone + ferrocyanide
-
 ?
1.1.5.9 D-glucose + oxidized 2,6-dichlorophenol indophenol
-
 Colletotrichum gloeosporioides D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.5.9 additional information suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview Colletotrichum gloeosporioides ?
-
 ?
1.1.5.9 additional information suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview Colletotrichum gloeosporioides DSM 62728 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 monomer 1 * 68000, deglycosylated enzyme, SDS-PAGE Colletotrichum gloeosporioides

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD-dependent glucose dehydrogenase
-
 Colletotrichum gloeosporioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.9 30
-
 assay at Colletotrichum gloeosporioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.9 5.5
-
 assay at with 2,6-dichlorophenol indophenol or ferrocenium hexafluorophosphate Colletotrichum gloeosporioides
1.1.5.9 7.5
-
 assay at with 1,4-benzoquinone Colletotrichum gloeosporioides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD dependent on, non-covalently bound Colletotrichum gloeosporioides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.5.9 Colletotrichum gloeosporioides isoelectric focusing
-
 5.6

General Information

EC Number General Information Comment Organism
1.1.5.9 evolution the enzyme belongs to the to the GMC family of oxidoreductases, phylogenetic analysis Colletotrichum gloeosporioides
1.1.5.9 physiological function the enzyme from Glomerella cingulata might play a role in plant pathogenicity. The quinone and phenoxy radical reducing enzyme light act to neutralize the action of plant laccase, phenoloxidase or peroxidase activities, which are increased in infected plants to evade fungal attack Colletotrichum gloeosporioides