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Literature summary extracted from

  • Sygmund, C.; Santner, P.; Krondorfer, I.; Peterbauer, C.K.; Alcalde, M.; Nyanhongo, G.S.; Guebitz, G.M.; Ludwig, R.
    Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production (2013), Microb. Cell Fact., 12, 38.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 expressed in Saccharomyces cerevisiae strain BJ5465 Thermothelomyces myriococcoides

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.18 N700S the kcat for oxygen turnover is increased in this mutant (4.5fold), but also substrate turnover. A 3fold increase of the kcat for cellobiose with alternative electron acceptors indicates that mutation N700S influences the oxidative- and reductive FAD half-reaction Thermothelomyces myriococcoides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.01147
-
 2,6-dichlorophenol indophenol recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.022
-
 1,4-benzoquinone recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.033
-
 2,6-dichlorophenol indophenol mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.037
-
 O2 recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.056
-
 1,4-benzoquinone mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.073
-
 O2 mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.18 cellobiose + 1,4-benzoquinone Thermothelomyces myriococcoides
-
 cellobiono-1,5-lactone + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol Thermothelomyces myriococcoides
-
 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellobiose + O2 Thermothelomyces myriococcoides
-
 cellobiono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Thermothelomyces myriococcoides A9XK88
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 phenyl Sepharose column chromatography and Q-Source column chromatography Thermothelomyces myriococcoides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 2.2
-
 culture supernatant, at pH 6.0 and 30°C Thermothelomyces myriococcoides
1.1.99.18 8
-
 after 3.6fold purification, at pH 6.0 and 30°C Thermothelomyces myriococcoides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 1,4-benzoquinone
-
 Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 1,4-benzoquinone
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Thermothelomyces myriococcoides cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellobiose + O2
-
 Thermothelomyces myriococcoides cellobiono-1,5-lactone + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 Cdh
-
 Thermothelomyces myriococcoides
1.1.99.18 cellobiose oxidase
-
 Thermothelomyces myriococcoides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.042
-
 O2 recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 0.19
-
 O2 mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 4.1
-
 2,6-dichlorophenol indophenol recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 12.4
-
 2,6-dichlorophenol indophenol mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 13.4
-
 1,4-benzoquinone mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 43
-
 1,4-benzoquinone recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
 Thermothelomyces myriococcoides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 1.1
-
 O2 recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 2.6
-
 O2 mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 185
-
 1,4-benzoquinone recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 240
-
 1,4-benzoquinone mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 360
-
 2,6-dichlorophenol indophenol recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides
1.1.99.18 375
-
 2,6-dichlorophenol indophenol mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C Thermothelomyces myriococcoides