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Literature summary extracted from

  • Zhang, W.; Shao, M.; Rao, Z.; Xu, M.; Zhang, X.; Yang, T.; Li, H.; Xu, Z.
    Bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Mycobacterium neoaurum JC-12 (2013), J. Steroid Biochem. Mol. Biol., 135, 36-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 gene ksdD, phylogenetic analysis, expression in Bacillus subtilis strain 168 with 18fold higher activity compared to wild-type Mycobacterium neoaurum strain JC-12 Mycolicibacterium neoaurum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + FAD Mycolicibacterium neoaurum
-
 1,4-androstadiene-3,17-dione + FADH2
-
 ?
1.3.99.4 4-androstene-3,17-dione + FAD Mycolicibacterium neoaurum JC-12
-
 1,4-androstadiene-3,17-dione + FADH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Mycolicibacterium neoaurum
-
 gene ksdD
-
1.3.99.4 Mycolicibacterium neoaurum JC-12
-
 gene ksdD
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.4 1.75
-
 recombinant enzyme expressed in Bacillus subtilis, pH 7.0, 30°C Mycolicibacterium neoaurum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + FAD
-
 Mycolicibacterium neoaurum 1,4-androstadiene-3,17-dione + FADH2
-
 ?
1.3.99.4 4-androstene-3,17-dione + FAD with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol Mycolicibacterium neoaurum 1,4-androstadiene-3,17-dione + FADH2
-
 ?
1.3.99.4 4-androstene-3,17-dione + FAD
-
 Mycolicibacterium neoaurum JC-12 1,4-androstadiene-3,17-dione + FADH2
-
 ?
1.3.99.4 4-androstene-3,17-dione + FAD with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol Mycolicibacterium neoaurum JC-12 1,4-androstadiene-3,17-dione + FADH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 3-ketosteroid-DELTA1-dehydrogenase
-
 Mycolicibacterium neoaurum
1.3.99.4 KsdD
-
 Mycolicibacterium neoaurum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.4 30
-
 assay at Mycolicibacterium neoaurum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.4 7
-
 assay at Mycolicibacterium neoaurum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.4 FAD putative N-terminal FAD-binding motif in KsdDM is GSG(A/G)(A/G)(A/G)X17E Mycolicibacterium neoaurum

General Information

EC Number General Information Comment Organism
1.3.99.4 metabolism the 3-ketosteroid-DELTA1-dehydrogenase is involved in steroid metabolism catalyzing the transformation of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione specifically Mycolicibacterium neoaurum