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Literature summary extracted from

  • Weia, T.; Fenga, S.; Shenb, Y.; Hea, P.; Maa, G.; Yua, X.; Zhang, F.; Mao, D.
    Characterization of a novel thermophilic pyrethroid-hydrolyzing carboxylesterase from Sulfolobus tokodaii into a new family (2013), J. Mol. Catal. B, 97, 225-232.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.1 degradation the enzyme can efficiently hydrolyze a wide range of synthetic pyrethroids including fenpropathrin, permethrin, cypermethrin, cyhalothrin, deltamethrin and bifenthrin, which makes it a potential candidate for the detoxification of pyrethroids for the purpose of biodegradation Sulfurisphaera tokodaii
3.1.1.1 environmental protection the enzyme can efficiently hydrolyze a wide range of synthetic pyrethroids including fenpropathrin, permethrin, cypermethrin, cyhalothrin, deltamethrin and bifenthrin, which makes it a potential candidate for the detoxification of pyrethroids for the purpose of biodegradation Sulfurisphaera tokodaii
3.1.1.88 degradation the enzyme can efficiently hydrolyze a wide range of synthetic pyrethroids including fenpropathrin, permethrin, cypermethrin, cyhalothrin, deltamethrin and bifenthrin, which makes it a potential candidate for the detoxification of pyrethroids for the purpose of biodegradation Sulfurisphaera tokodaii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 overexpressed in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 acetone 50% (v/v), 2 h incubation, 60% loss of activity Sulfurisphaera tokodaii
3.1.1.1 chloroform 50% (v/v), 2 h incubation, 66% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Co2+ 5 mM, 38% inhibition Sulfurisphaera tokodaii
3.1.1.1 Cu2+ 5 mM, 57% inhibition; 5 mM, reduces the esterase activity to 43% Sulfurisphaera tokodaii
3.1.1.1 diethyldicarbonate 5 mM,% inhibition Sulfurisphaera tokodaii
3.1.1.1 diethylpyrocarbonate 5 mM,% inhibition Sulfurisphaera tokodaii
3.1.1.1 dimethylformamide 50% (v/v), 2 h incubation, 72% loss of activity Sulfurisphaera tokodaii
3.1.1.1 DTT 5 mM,% inhibition Sulfurisphaera tokodaii
3.1.1.1 EDTA 5 mM, 17% inhibition Sulfurisphaera tokodaii
3.1.1.1 ethanol 50% (v/v), 2 h incubation, 67% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Fe2+ 5 mM, 81% inhibition; 5 mM, reduces the esterase activity to 19% Sulfurisphaera tokodaii
3.1.1.1 formaldehyde 20% (v/v), 2 h incubation, 81% loss of activity Sulfurisphaera tokodaii
3.1.1.1 heptane 20% (v/v), 2 h incubation, 88% loss of activity Sulfurisphaera tokodaii
3.1.1.1 methanol 50% (v/v), 2 h incubation, 58% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Mn2+ 5 mM, 27% inhibition Sulfurisphaera tokodaii
3.1.1.1 n-hexane 20% (v/v), 2 h incubation, 67% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Ni2+ 5 mM, 22% inhibition Sulfurisphaera tokodaii
3.1.1.1 PMSF 5 mM, complete inhibition Sulfurisphaera tokodaii
3.1.1.1 Propanol 50% (v/v), 2 h incubation, 59% loss of activity Sulfurisphaera tokodaii
3.1.1.1 SDS 5% (w/v), 90% inhibition Sulfurisphaera tokodaii
3.1.1.1 Toluene 20% (v/v), 2 h incubation, 86% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Triton X-100 5% (w/v), 42% inhibition Sulfurisphaera tokodaii
3.1.1.1 Tween 20 5% (w/v), 28% inhibition Sulfurisphaera tokodaii
3.1.1.1 Tween 80 5% (w/v), 38% inhibition Sulfurisphaera tokodaii
3.1.1.1 Zn2+ 5 mM, 87% inhibition; 5 mM, reduces the esterase activity to 13% Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.005
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.0051
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.009
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.0094
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.015
-
 4-nitrophenyl dodecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.033
-
 4-nitrophenyl decanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.152
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.153
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.239
-
 4-nitrophenyl butyrate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.464
-
 4-nitrophenyl acetate pH 9.0, 80°C Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 additional information 5 mM of various metal ions (Ca2+, Mg2+, Mn2+, Co2+,Ni2+, Na+, and K+) do not significantly affect the enzymatic activity after incubation for 60 min at room temperature Sulfurisphaera tokodaii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 37000
-
 x * 37000, SDS-PAGE Sulfurisphaera tokodaii
3.1.1.1 37400
-
 x * 37400, calculated from sequence Sulfurisphaera tokodaii

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.1 Acetone 50% (v/v), 2 h incubation, 60% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 Acetone 50% (v/v), pH 9.0, 30°C, 60% loss of activity Sulfurisphaera tokodaii
3.1.1.1 chloroform 50% (v/v), 2 h incubation, 66% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 chloroform 50% (v/v), pH 9.0, 30°C, 66% loss of activity Sulfurisphaera tokodaii
3.1.1.1 dimethyl formamide 50% (v/v), 2 h incubation, 72% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 dimethylformamide 50% (v/v), pH 9.0, 30°C, 72% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Ethanol 50% (v/v), 2 h incubation, 67% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 Ethanol 50% (v/v), pH 9.0, 30°C, 67% loss of activity Sulfurisphaera tokodaii
3.1.1.1 formaldehyde 20% (v/v), 2 h incubation, 81% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 formaldehyde 50% (v/v), pH 9.0, 30°C, 81% loss of activity Sulfurisphaera tokodaii
3.1.1.1 heptane 20% (v/v), 2 h incubation, 88% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 heptane 50% (v/v), pH 9.0, 30°C, 88% loss of activity Sulfurisphaera tokodaii
3.1.1.1 Methanol 50% (v/v), 2 h incubation, 58% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 Methanol 50% (v/v), pH 9.0, 30°C, 58% loss of activity Sulfurisphaera tokodaii
3.1.1.1 n-hexane 20% (v/v), 2 h incubation, 67% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 n-hexane 50% (v/v), pH 9.0, 30°C, 77% loss of activity Sulfurisphaera tokodaii
3.1.1.1 propanol 50% (v/v), 2 h incubation, 59% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 propanol 50% (v/v), pH 9.0, 30°C, 59% loss of activity Sulfurisphaera tokodaii
3.1.1.1 toluene 20% (v/v), 2 h incubation, 86% loss of activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii
3.1.1.1 toluene 50% (v/v), pH 9.0, 30°C, 86% loss of activity Sulfurisphaera tokodaii

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Sulfurisphaera tokodaii Q96Z02
-
-
3.1.1.1 Sulfurisphaera tokodaii 7 Q96Z02
-
-
3.1.1.88 Sulfurisphaera tokodaii Q96Z02
-
-
3.1.1.88 Sulfurisphaera tokodaii 7 Q96Z02
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 13
-
 substrate: 4-nitrophenyl hexadecanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 19
-
 substrate: 4-nitrophenyl tetradecanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 37
-
 substrate: 4-nitrophenyl dodecanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 56
-
 substrate: 4-nitrophenyl decanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 189
-
 substrate: 4-nitrophenyl octanoate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 213
-
 substrate: 4-nitrophenyl acetate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 354
-
 substrate: 4-nitrophenyl butyrate, pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 3542
-
 pH 9.0, 80°C, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O most preferable substrate Sulfurisphaera tokodaii 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O most preferable substrate Sulfurisphaera tokodaii 7 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexadecanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + hexadecanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Sulfurisphaera tokodaii 7 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl tetradecanoate + H2O
-
 Sulfurisphaera tokodaii 4-nitrophenol + tetradecanoate
-
 ?
3.1.1.88 bifenthrin + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.1.1.88 cyhalothrin + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.1.1.88 cypermethrin + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.1.1.88 deltamethrin + H2O
-
 Sulfurisphaera tokodaii 3-phenoxybenzaldehyde + (1R,3R)-3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropanecarboxylate + cyanide
-
 ?
3.1.1.88 fenpropathrin + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?
3.1.1.88 permethrin + H2O
-
 Sulfurisphaera tokodaii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 37000, SDS-PAGE Sulfurisphaera tokodaii
3.1.1.1 ? x * 37400, calculated from sequence Sulfurisphaera tokodaii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 EstSt7
-
 Sulfurisphaera tokodaii
3.1.1.1 ST2026
-
 Sulfurisphaera tokodaii
3.1.1.1 ST2026 locus name Sulfurisphaera tokodaii
3.1.1.88 EstSt7
-
 Sulfurisphaera tokodaii
3.1.1.88 ST2026 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 80
-
-
 Sulfurisphaera tokodaii
3.1.1.1 80
-
 substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 50 95 50°C: about 40% of maximal activity, 95°C: about 50% of maximal activity Sulfurisphaera tokodaii
3.1.1.1 50 95 50°C: about 40% of maximal activity, 95°C: about 50% of maximal activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 70
-
 stable for at least 240 min Sulfurisphaera tokodaii
3.1.1.1 90
-
 half-life: 180 min Sulfurisphaera tokodaii
3.1.1.88 90
-
 half-life: 180 min Sulfurisphaera tokodaii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 0.03
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.11
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 0.2
-
 4-nitrophenyl dodecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 1.5
-
 4-nitrophenyl decanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 21.1
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 58.9
-
 4-nitrophenyl butyrate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 74.6
-
 4-nitrophenyl acetate pH 9.0, 80°C Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9
-
-
 Sulfurisphaera tokodaii
3.1.1.1 9
-
 substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 7 11 pH 7.0: about 40% of maximal activity, pH 11: about 40% of maximal activity, substrate: 4-nitrophenyl butyrate Sulfurisphaera tokodaii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 8 10 80°C, 60 min, 80% of maximal activity Sulfurisphaera tokodaii
3.1.1.1 8 10 80°C, 60 min, the enzyme maintains over 80% of its maximal activity Sulfurisphaera tokodaii
3.1.1.88 8 10 80°C, 60 min, the enzyme maintains over 80% of its maximal activity Sulfurisphaera tokodaii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 5.9
-
 4-nitrophenyl hexadecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 11.7
-
 4-nitrophenyl tetradecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 13.5
-
 4-nitrophenyl dodecanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 45.7
-
 4-nitrophenyl decanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 138
-
 4-nitrophenyl octanoate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 160.7
-
 4-nitrophenyl acetate pH 9.0, 80°C Sulfurisphaera tokodaii
3.1.1.1 246.3
-
 4-nitrophenyl butyrate pH 9.0, 80°C Sulfurisphaera tokodaii