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Literature summary extracted from

  • Juneja, P.; Hubrich, F.; Diederichs, K.; Welte, W.; Andexer, J.N.
    Mechanistic implications for the chorismatase FkbO based on the crystal structure (2014), J. Mol. Biol., 426, 105-115.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.13 expressed in Escherichia coli BL21-RPpL1SL2(DE3) cells Streptomyces hygroscopicus subsp. ascomyceticus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.3.2.13 in complex with 3-(2-carboxyethyl)benzoate, hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 20-25% (w/v) polyethylene glycol 4000 Streptomyces hygroscopicus subsp. ascomyceticus

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.13 E338A inactive Streptomyces hygroscopicus subsp. ascomyceticus
3.3.2.13 E338Q inactive Streptomyces hygroscopicus subsp. ascomyceticus
3.3.2.13 F226A the mutant shows 2.5% activity compared to the wild type enzyme Streptomyces hygroscopicus subsp. ascomyceticus
3.3.2.13 R228A the mutant shows 224.5% activity compared to the wild type enzyme Streptomyces hygroscopicus subsp. ascomyceticus
3.3.2.13 Y215F the mutant shows 12% activity compared to the wild type enzyme Streptomyces hygroscopicus subsp. ascomyceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.13 3-(2-carboxyethyl)benzoate competitive inhibitor Streptomyces hygroscopicus subsp. ascomyceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.13 0.15
-
 chorismate wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25°C Streptomyces hygroscopicus subsp. ascomyceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.13 chorismate + H2O Streptomyces hygroscopicus subsp. ascomyceticus
-
 (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.13 Streptomyces hygroscopicus subsp. ascomyceticus Q9KID9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.13 Superdex 75 gel filtration Streptomyces hygroscopicus subsp. ascomyceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.13 chorismate + H2O
-
 Streptomyces hygroscopicus subsp. ascomyceticus (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.13 fkbO
-
 Streptomyces hygroscopicus subsp. ascomyceticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3.2.13 1.02
-
 3-(2-carboxyethyl)benzoate wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25°C Streptomyces hygroscopicus subsp. ascomyceticus