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Literature summary extracted from
- Reversible unfolding of a thermophilic membrane protein in phospholipid/detergent mixed micelles (2010), J. Mol. Biol., 397, 550-559.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.2.2.8 | expression in Escherichia coli | Archaeoglobus fulgidus |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 7.2.2.8 | guanidine-HCl | the incubation of the enzyme reconstituted in phospholipid/detergent mixed micelles with high concentrations of guanidinium hydrochloride at 25°C induces a reversible decrease in fluorescence quantum yield, far-UV ellipticity, and loss of ATPase and phosphatase activities. Refolding of the enzyme from this unfolded state leads to recovery of full biological activity and all the structural features of the native enzyme. The unfolding shows typical characteristics of a two-state process. Most of the secondary and tertiary structures are disrupted. The fraction of Trp fluorescence accessible to soluble quenchers shifts from 0.52 in the native state to 0.96 in the unfolded state, with a significant spectral redshift. Hydrophobic patches in the enzyme, mainly located in the transmembrane region, are disrupted as indicated by 1-anilino-naphtalene-8-sulfonate fluorescence. Nevertheless, the unfolded state has a small but detectable amount of residual structure, which might play a key role in both CopA folding and adaptation for working at high temperatures | Archaeoglobus fulgidus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.8 | Archaeoglobus fulgidus | O29777 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.2.2.8 | - |
Archaeoglobus fulgidus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.2.2.8 | CopA | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.8 | 75 | - |
assay at | Archaeoglobus fulgidus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.8 | 6.1 | - |
assay | Archaeoglobus fulgidus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.2.2.8 | physiological function | the enzyme is involved in transporting Cu+ throughout biological membranes | Archaeoglobus fulgidus |
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Release 2023.1 (January 2023)
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