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Literature summary extracted from

  • Suzuki, Y.; Noma, A.; Suzuki, T.; Senda, M.; Senda, T.; Ishitani, R.; Nureki, O.
    Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA (2007), J. Mol. Biol., 372, 1204-1214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.44
-
 Methanocaldococcus jannaschii
4.1.3.44
-
 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.3.44 to 2.4 A resolution. Enzyme TYW1 assumes an incomplete TIM barrel with (alpha/beta)6 topology, which closely resembles the reported structures of radical SAM enzymes. TYW1 is considered to catalyze the cyclization reaction by utilizing the radical intermediate. construction of a model structure complexed with S-adenosylmethionine and two [4Fe-4S] clusters Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.44 C444S loss of activity Saccharomyces cerevisiae
4.1.3.44 C457S loss of activity Saccharomyces cerevisiae
4.1.3.44 C470S loss of activity Saccharomyces cerevisiae
4.1.3.44 C479S loss of activity Saccharomyces cerevisiae
4.1.3.44 C483S loss of activity Saccharomyces cerevisiae
4.1.3.44 C486S loss of activity Saccharomyces cerevisiae
4.1.3.44 D597A loss of activity Saccharomyces cerevisiae
4.1.3.44 D597N loss of activity Saccharomyces cerevisiae
4.1.3.44 E550A loss of activity Saccharomyces cerevisiae
4.1.3.44 K459A loss of activity Saccharomyces cerevisiae
4.1.3.44 R638A loss of activity Saccharomyces cerevisiae
4.1.3.44 S595A loss of activity Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.44 Methanocaldococcus jannaschii Q57705
-
-
4.1.3.44 Methanocaldococcus jannaschii DSM 2661 Q57705
-
-
4.1.3.44 Saccharomyces cerevisiae Q08960
-
-
4.1.3.44 Saccharomyces cerevisiae ATCC 204508 Q08960
-
-