EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.2.4 | recombinant enzyme expression in Escherichia coli strain DH5alpha | Malassezia globosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.4 | additional information | - |
additional information | steady-state kinetics of P450 enzyme reactions supported by Malassezia globosa NPR, overview | Malassezia globosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.2.4 | membrane | - |
Malassezia globosa | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 80740 | - |
x * 80740, SDS-PAGE | Malassezia globosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.4 | NADPH + H+ + cytochrome c | Malassezia globosa | - |
NADP+ + reduced cytochrome c | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.4 | Malassezia globosa | A8PYK0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.4 | recombinant enzyme from Escherichia coli strain DH5alpha | Malassezia globosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.4 | additional information | NADPH-dependent reducing activity for cytochrome c or nitroblue tetrazolium | Malassezia globosa | ? | - |
? | |
1.6.2.4 | NADPH + H+ + cytochrome c | - |
Malassezia globosa | NADP+ + reduced cytochrome c | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.6.2.4 | ? | x * 80740, SDS-PAGE | Malassezia globosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.6.2.4 | NADPH-P450 reductase | - |
Malassezia globosa |
1.6.2.4 | NPR | - |
Malassezia globosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 37 | - |
assay at | Malassezia globosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 7.4 | - |
assay at | Malassezia globosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.4 | flavin | - |
Malassezia globosa | |
1.6.2.4 | NADPH | - |
Malassezia globosa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.6.2.4 | metabolism | human P450 1A2 and 2A6 are able to successfully catalyze the O-deethylation of 7-ethoxyresorufin and the 7-hydroxylation of coumarin, respectively, with the support of the purified enzyme NPR. Purified NPR is an orthologous reductase protein that supports cytochrome P450 enzymes in Malassezia globosa | Malassezia globosa |