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Literature summary extracted from
- Purification and characterization of protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707 (2013), J. Korean Soc. Appl. Biol. Chem., 56, 401-408.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.3 | additional information | - |
additional information | the enzyme exhibits Michaelis-Menten kinetics for all substrates tested | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.002 | - |
gallate | pH 7.5, 38°C | Pseudomonas oleovorans |  |
| 1.13.11.3 | 0.014 | - |
catechol | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.014 | - |
L-Dopa | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.014 | - |
4-methylcatechol | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.014 | - |
caffeic acid | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.014 | - |
pyrogallol | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.014 | - |
catechin | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.014 | - |
4-Cresol | pH 7.5, 38°C | Pseudomonas oleovorans | |
| 1.13.11.3 | 0.017 | - |
3,4-dihydroxybenzoate | pH 7.5, 38°C | Pseudomonas oleovorans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 29000 | - |
trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE | Pseudomonas oleovorans |
| 1.13.11.3 | 34300 | - |
trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE | Pseudomonas oleovorans |
| 1.13.11.3 | 189900 | - |
gel filtration | Pseudomonas oleovorans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | Pseudomonas oleovorans | - |
3-carboxy-cis,cis-muconate | - |
? | |
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | Pseudomonas oleovorans KF707 | - |
3-carboxy-cis,cis-muconate | - |
? | |
| 1.13.11.3 | additional information | Pseudomonas oleovorans | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | ? | - |
? | |
| 1.13.11.3 | additional information | Pseudomonas oleovorans KF707 | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.3 | Pseudomonas oleovorans | - |
- |
- |
| 1.13.11.3 | Pseudomonas oleovorans KF707 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.3 | native enzyme 296.8fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography | Pseudomonas oleovorans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 121.7 | - |
purified native enzyme, pH 7.5, 38°C | Pseudomonas oleovorans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas oleovorans | 3-carboxy-cis,cis-muconate | - |
? | |
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | i.e. protocatechuate | Pseudomonas oleovorans | 3-carboxy-cis,cis-muconate | - |
? | |
| 1.13.11.3 | 3,4-dihydroxybenzoate + O2 | - |
Pseudomonas oleovorans KF707 | 3-carboxy-cis,cis-muconate | - |
? | |
| 1.13.11.3 | 4-cresol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | 4-methylcatechol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | caffeic acid + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | caffeic acid + O2 | - |
Pseudomonas oleovorans KF707 | ? | - |
? | |
| 1.13.11.3 | catechin + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | catechol + O2 | reaction of EC 1.13.11.1 | Pseudomonas oleovorans | cis,cis-muconate | - |
? | |
| 1.13.11.3 | catechol + O2 | reaction of EC 1.13.11.1 | Pseudomonas oleovorans KF707 | cis,cis-muconate | - |
? | |
| 1.13.11.3 | gallate + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | L-DOPA + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | additional information | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | additional information | the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources | Pseudomonas oleovorans KF707 | ? | - |
? | |
| 1.13.11.3 | pyrogallol + O2 | - |
Pseudomonas oleovorans | ? | - |
? | |
| 1.13.11.3 | pyrogallol + O2 | - |
Pseudomonas oleovorans KF707 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.3 | hexamer | trimer of dimers, (alphabeta)3, 3 * 29000, alpha subunit, + 3 * 34300, beta-subunit, SDS-PAGE | Pseudomonas oleovorans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 38 | - |
- |
Pseudomonas oleovorans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 20 | 50 | purified enzyme, 50 min, pH 7.5, relatively stable | Pseudomonas oleovorans |
| 1.13.11.3 | 70 | - |
purified enzyme, 20 min, pH 7.5, loss of 95% activity | Pseudomonas oleovorans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.3 | 7.5 | - |
- |
Pseudomonas oleovorans |
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