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Literature summary extracted from

  • Dong, Q.; Yan, X.; Zheng, M.; Yang, Z.
    Characterization of an extremely thermostable but cold-adaptive beta-galactosidase from the hyperthermophilic archaeon Pyrococcus furiosus for use as a recombinant aggregation for batch lactose degradation at high temperature (2014), J. Biosci. Bioeng., 117, 706-710.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 food industry the recombinant thermostable beta-galactosidase may be suitable for the hydrolysis of lactose in milk processing Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli BL21. The active inclusion bodies of beta-galactosidase are easily isolated by nonionic detergent treatment and directly used for lactose conversion in a repetitive batch mode Pyrococcus furiosus

General Stability

EC Number General Stability Organism
3.2.1.23 when the recombinant enzyme from active inclusion bodies is utilized for lactose conversion in a repetitive batch mode, more than 54% (90°C) or 88% (10°C) of the original enzyme activity is retained after 10 conversion cycles under optimum conditions Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ca2+ 5 mM, inhibits the enzyme activity by 22.7%, 80°C, pH 7.0 Pyrococcus furiosus
3.2.1.23 Cu2+ 5 mM, inhibits the enzyme activity by 99.71%, 80°C, pH 7.0 Pyrococcus furiosus
3.2.1.23 Fe3+ 5 mM, inhibits the enzyme activity by 95.2%, 80°C, pH 7.0 Pyrococcus furiosus
3.2.1.23 Mn2+ 5 mM, inhibits the enzyme activity by 24.8%, 80°C, pH 7.0 Pyrococcus furiosus
3.2.1.23 Ni2+ 5 mM, inhibits the enzyme activity by 92.9%, 80°C, pH 7.0 Pyrococcus furiosus
3.2.1.23 Zn2+ 5 mM, inhibits the enzyme activity by 97.3%, 80°C, pH 7.0 Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Mg2+ 5 mM, 1.1fold stimulation of activity, 80°C, pH 7.0 Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 59450
-
 x * 59450, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Pyrococcus furiosus E7FHC0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 10.2
-
 pH 7, 0°C Pyrococcus furiosus
3.2.1.23 130
-
 pH 7, 90°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O 94% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside Pyrococcus furiosus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.23 lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 ?
3.2.1.23 additional information activity with 4-nitrophenyl alpha-D-glucopyranoside is 0.26% of the activity compared to 2-nitrophenyl beta-D-galactopyranoside, activity with 4-nitrophenyl alpha-D-glucopyranoside is 10.91% of the activity compared to 2-nitrophenyl beta-D-galactopyranoside Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 59450, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 lactase
-
 Pyrococcus furiosus
3.2.1.23 PF1208 locus name Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 60 100 60°C: about 50% of maximal activity, 100°C: about 95% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 70
-
 pH 7.0, half-life: 31423.4 min Pyrococcus furiosus
3.2.1.23 80
-
 pH 7.0, half-life: 8168.3 min Pyrococcus furiosus
3.2.1.23 85
-
 pH 7.0, half-life: 4017.7 min Pyrococcus furiosus
3.2.1.23 90
-
 pH 7.0, half-life: 547.4 min Pyrococcus furiosus
3.2.1.23 95
-
 pH 7.0, half-life: 309.6 min Pyrococcus furiosus
3.2.1.23 100
-
 pH 7.0, half-life: 203.5 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
 in phosphate buffer Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 6.5 7.5 pH 6.5: about 60% of maximal activity, pH 7.5: about 50% of maximal activity Pyrococcus furiosus