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Literature summary extracted from
- Semiquinone and cluster N6 signals in His-tagged proton-translocating NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli (2013), J. Biol. Chem., 288, 14310-14319.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.1.1.2 | expression of His-tagged complex I | Escherichia coli |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | Fe2+ | Fe-S cluster, 29.0-0.37 of iron/1 mol of complex I | Escherichia coli |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 570000 | - |
about, recombinant His-tagged complex I, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | Escherichia coli | - |
NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.2 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.2 | recombinant His-tagged complex I by nickel affinity chromatography and gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.2 | NADH + ubiquinone + 6 H+[side 1] | - |
Escherichia coli | NAD+ + ubiquinol + 7 H+[side 2] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | More | complex I consists of 13 different subunits named NuoA-Nand encoded by the nuo operon | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | complex I | - |
Escherichia coli |
| 7.1.1.2 | proton-translocating NADH:ubiquinone oxidoreductase | - |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 7.5 | - |
assay at | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | FMN | 0.94-0.1 of FMN//1 mol of complex I | Escherichia coli | |
| 7.1.1.2 | NADH | - |
Escherichia coli | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | physiological function | NADH:ubiquinone oxidoreductase (complex I) pumps protons across the membrane using downhill redox energy | Escherichia coli |
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