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Literature summary extracted from

  • Sakuraba, H.; Satomura, T.; Kawakami, R.; Kim, K.; Hara, Y.; Yoneda, K.; Ohshima, T.
    Crystal structure of novel dye-linked L-proline dehydrogenase from hyperthermophilic archaeon Aeropyrum pernix (2012), J. Biol. Chem., 287, 20070-20080.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 expressed in Escherichia coli BL21 (DE3) Codon Plus RILP cells Aeropyrum pernix

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.99.B2 enzyme bound with L-proline, sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M trisodium citrate dihydrate (pH 5.6), and 30% (w/v) polyethylene glycol 4000 Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.B2 0.28
-
L-proline wild type enzyme, at pH 8.0 and 50°C Aeropyrum pernix
1.5.99.B2 230
-
L-proline DELTAL428 mutant enzyme, at pH 8.0 and 50°C Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.B2 18000
-
2 * 18000 Aeropyrum pernix
1.5.99.B2 36000
-
-
Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Aeropyrum pernix
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2 Ni2+-charged chelating Sepharose column chromatography and Sephacryl S-300 gel filtration Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O
-
Aeropyrum pernix DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
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Subunits

EC Number Subunits Comment Organism
1.5.99.B2 homodimer 2 * 18000 Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 L-proline dehydrogenase
-
Aeropyrum pernix
1.5.99.B2 LPDH
-
Aeropyrum pernix

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD
-
Aeropyrum pernix