EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.B2 | expressed in Escherichia coli BL21 (DE3) Codon Plus RILP cells | Aeropyrum pernix |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.99.B2 | enzyme bound with L-proline, sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M trisodium citrate dihydrate (pH 5.6), and 30% (w/v) polyethylene glycol 4000 | Aeropyrum pernix |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.B2 | 0.28 | - |
L-proline | wild type enzyme, at pH 8.0 and 50°C | Aeropyrum pernix | |
1.5.99.B2 | 230 | - |
L-proline | DELTAL428 mutant enzyme, at pH 8.0 and 50°C | Aeropyrum pernix |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 18000 | - |
2 * 18000 | Aeropyrum pernix |
1.5.99.B2 | 36000 | - |
- |
Aeropyrum pernix |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.B2 | Aeropyrum pernix | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.99.B2 | Ni2+-charged chelating Sepharose column chromatography and Sephacryl S-300 gel filtration | Aeropyrum pernix |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.B2 | L-proline + 2,6-dichlorophenolindophenol + H2O | - |
Aeropyrum pernix | DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.99.B2 | homodimer | 2 * 18000 | Aeropyrum pernix |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.B2 | L-proline dehydrogenase | - |
Aeropyrum pernix |
1.5.99.B2 | LPDH | - |
Aeropyrum pernix |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.B2 | FAD | - |
Aeropyrum pernix |