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Literature summary extracted from

  • Broecker, M.J.; Waetzlich, D.; Saggu, M.; Lendzian, F.; Moser, J.; Jahn, D.
    Biosynthesis of (bacterio)chlorophylls: ATP-dependent transient subunit interaction and electron transfer of dark operative protochlorophyllide oxidoreductase (2010), J. Biol. Chem., 285, 8268-8277.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.7 additional information the ternary DPOR enzyme holocomplex comprising subunits ChlN, ChlB, and ChlL is trapped as an octameric (ChlN/ChlB)2(ChlL2)2 complex after incubation with the nonhydrolyzable ATP analogues adenosine 5'-(gamma-thio)triphosphate, adenosine 5'-(beta,gamma-imido)triphosphate, or MgADP in combination with AlF4-, complex structure, overview. A mutant ChlL2 protein, with a deleted Leu153 in the switch II region also allows for the formation of a stable octameric complex Prochlorococcus marinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.7.7 Fe2+ the homodimeric ChlL2 subunit carries a [4Fe-4S] cluster Prochlorococcus marinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.7.7 35000
-
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation Prochlorococcus marinus
1.3.7.7 45000
-
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation Prochlorococcus marinus
1.3.7.7 46199
-
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation Prochlorococcus marinus
1.3.7.7 58729
-
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation Prochlorococcus marinus
1.3.7.7 60000
-
octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation Prochlorococcus marinus
1.3.7.7 360000
-
enzyme complex, gel filtration Prochlorococcus marinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.7 additional information Prochlorococcus marinus proposed catalytic redox cycle of DPOR, overview ?
-
?
1.3.7.7 protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O Prochlorococcus marinus
-
chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.7 Prochlorococcus marinus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.7 additional information proposed catalytic redox cycle of DPOR, overview Prochlorococcus marinus ?
-
?
1.3.7.7 protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O
-
Prochlorococcus marinus chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate
-
?
1.3.7.7 protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O the homodimeric ChlL2 subunit carrying a [4Fe-4S] cluster transfers electrons to the corresponding heterotetrameric catalytic subunit (ChlN/ChlB)2, which also possesses a redox active [4Fe-4S] cluster Prochlorococcus marinus chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate
-
?

Subunits

EC Number Subunits Comment Organism
1.3.7.7 octamer octameric (ChlN/ChlB)2(ChlL2)2 subunit complex, 4 * 35000, subunit ChlL, + 2 * 45000, subunit ChlN, 2 * 60000, subunit ChlB, SDS-PAGE, 4 * 32395,subunit ChlL, + 2 * 46199, subunit ChlN, 2 * 58729, subunit ChlB, sequence calculation Prochlorococcus marinus

Synonyms

EC Number Synonyms Comment Organism
1.3.7.7 dark operative protochlorophyllide oxidoreductase
-
Prochlorococcus marinus
1.3.7.7 DPOR
-
Prochlorococcus marinus
1.3.7.7 protochlorophyllide oxidoreductase
-
Prochlorococcus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.7.7 25
-
assay at Prochlorococcus marinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.7 ATP
-
Prochlorococcus marinus
1.3.7.7 Ferredoxin
-
Prochlorococcus marinus

General Information

EC Number General Information Comment Organism
1.3.7.7 additional information transient protein-protein interaction of ChlL2 and (ChlN/ChlB)2 is essential for the ATP-dependent electron transfer processes catalyzed by DPOR. Efficient octameric (ChlN/ChlB)2(ChlL2)2 enzyme complex formation required the presence of protochlorophyllide. Complete ATP hydrolysis is a prerequisite for intersubunit electron transfer Prochlorococcus marinus