Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gruez, A.; Libiad, M.; Boschi-Muller, S.; Branlant, G.
    Structural and biochemical characterization of free methionine-R-sulfoxide reductase from Neisseria meningitidis (2010), J. Biol. Chem., 285, 25033-25043.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.14 expression in Escherichia coli Neisseria meningitidis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.14 in complex with methionine sulfoxide, to 1.25 A resolution. Residue C118 is the catalytic Cys on which a sulfenic acid is formed Neisseria meningitidis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.14 C118A inactive Neisseria meningitidis
1.8.4.14 C136A about 60% of the catalytic efficiency of wild-type Neisseria meningitidis
1.8.4.14 C84A about 120% of the catalytic efficiency of wild-type Neisseria meningitidis
1.8.4.14 C84A/C136A catalytic efficiency similar to wild-type Neisseria meningitidis
1.8.4.14 C84A/C94A/C136A about 35% of the catalytic efficiency of wild-type Neisseria meningitidis
1.8.4.14 C94A about 40% of the catalytic efficiency of wild-type Neisseria meningitidis
1.8.4.14 C94A/C136A about 35% of the catalytic efficiency of wild-type Neisseria meningitidis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.14 0.015
-
 thioredoxin mutant C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 0.016
-
 thioredoxin mutant C84A/C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 0.024
-
 thioredoxin mutant C94A , 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 0.027
-
 thioredoxin mutant C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 0.04
-
 thioredoxin mutant C84A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 0.04
-
 thioredoxin wild-type, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 0.05
-
 thioredoxin mutant C84A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 2
-
 L-methionine (R)-S-oxide wild-type, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 2.2
-
 L-methionine (R)-S-oxide mutant C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 12
-
 L-methionine (R)-S-oxide mutant C84A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 18
-
 L-methionine (R)-S-oxide mutant C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 30
-
 L-methionine (R)-S-oxide mutant C84A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 30
-
 L-methionine (R)-S-oxide mutant C84A/C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 32
-
 L-methionine (R)-S-oxide mutant C94A , 25°C, pH 8.0 Neisseria meningitidis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.14 18166
-
 2 * 18166, calculated Neisseria meningitidis
1.8.4.14 36330
-
 mass spectrometry under non-denaturing conditions Neisseria meningitidis

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.14 Neisseria meningitidis A0A0Y6TLH6 serogroup C
-
1.8.4.14 Neisseria meningitidis 8013 A0A0Y6TLH6 serogroup C
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.14 L-methionine (R)-S-oxide + thioredoxin
-
 Neisseria meningitidis L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.14 L-methionine (R)-S-oxide + thioredoxin
-
 Neisseria meningitidis 8013 L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.14 additional information in the absence of thioredoxin, 1 mol of Met/subunit is formed Neisseria meningitidis ?
-
 ?
1.8.4.14 additional information in the absence of thioredoxin, 1 mol of Met/subunit is formed Neisseria meningitidis 8013 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.14 dimer 2 * 18166, calculated Neisseria meningitidis

Synonyms

EC Number Synonyms Comment Organism
1.8.4.14 NMV_2074 locus name Neisseria meningitidis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.14 1
-
 L-methionine (R)-S-oxide mutant C84A/C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 1
-
 L-methionine (R)-S-oxide mutant C94A , 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 2.6
-
 L-methionine (R)-S-oxide mutant C84A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 8.5
-
 L-methionine (R)-S-oxide mutant C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 13
-
 L-methionine (R)-S-oxide mutant C94A/C136A, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 14
-
 L-methionine (R)-S-oxide wild-type, 25°C, pH 8.0 Neisseria meningitidis
1.8.4.14 18
-
 L-methionine (R)-S-oxide mutant C84A, 25°C, pH 8.0 Neisseria meningitidis