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Literature summary extracted from

  • Guo, Q.; Gong, Q.; Tong, K.L.; Vestergaard, B.; Costa, A.; Desgres, J.; Wong, M.; Grosjean, H.; Zhu, G.; Wong, J.T.; Xue, H.
    Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains (2002), J. Biol. Chem., 277, 14343-14349.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.2 expression in Escherichia coli Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.2 0.00005
-
 Archeoglobus fulgidus tRNATrp(A73C) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00005
-
 Archeoglobus fulgidus tRNATrp(A73U) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00006
-
 Archeoglobus fulgidus tRNATrp(A73, wild-type) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00006
-
 Archeoglobus fulgidus tRNATrp(A73G) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00009
-
 Bacillus subtilis tRNATrp(A73, wild-type) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.0001
-
 Bacillus subtilis tRNATrp(A73G) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00012
-
 Bacillus subtilis tRNATrp(A73C) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00012
-
 Bacillus subtilis tRNATrp(A73U) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00017
-
 bovine tRNATrp(G73U) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00018
-
 bovine tRNATrp(G73, wild-type) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00021
-
 bovine tRNATrp(G73A) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.00022
-
 bovine tRNATrp(G73C) pH 7.0, 65°C Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.2 Archaeoglobus fulgidus O28579
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.2 ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73, wild-type) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73, wild-type)
-
 ?
6.1.1.2 ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73C) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73C)
-
 ?
6.1.1.2 ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73G) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73G)
-
 ?
6.1.1.2 ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73U) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73U)
-
 ?
6.1.1.2 ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73, wild-type) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73, wild-type)
-
 ?
6.1.1.2 ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73C) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73C)
-
 ?
6.1.1.2 ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73G) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73G)
-
 ?
6.1.1.2 ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73U) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73U)
-
 ?
6.1.1.2 ATP + L-tryptophan + bovine tRNATrp(G73, wild-type) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73, wild-type)
-
 ?
6.1.1.2 ATP + L-tryptophan + bovine tRNATrp(G73A) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73A)
-
 ?
6.1.1.2 ATP + L-tryptophan + bovine tRNATrp(G73C) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73C)
-
 ?
6.1.1.2 ATP + L-tryptophan + bovine tRNATrp(G73U) preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp) Archaeoglobus fulgidus AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73U)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.2 TrpRS
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.2 65
-
 assay at Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.2 0.03
-
 bovine tRNATrp(G73, wild-type) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.04
-
 bovine tRNATrp(G73U) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.04
-
 Bacillus subtilis tRNATrp(A73G) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.05
-
 Bacillus subtilis tRNATrp(A73U) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.06
-
 bovine tRNATrp(G73C) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.06
-
 Bacillus subtilis tRNATrp(A73C) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.07
-
 Archeoglobus fulgidus tRNATrp(A73G) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.08
-
 bovine tRNATrp(G73A) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.09
-
 Archeoglobus fulgidus tRNATrp(A73U) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.11
-
 Archeoglobus fulgidus tRNATrp(A73C) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.14
-
 Bacillus subtilis tRNATrp(A73, wild-type) pH 7.0, 65°C Archaeoglobus fulgidus
6.1.1.2 0.53
-
 Archeoglobus fulgidus tRNATrp(A73, wild-type) pH 7.0, 65°C Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.2 7
-
 assay at Archaeoglobus fulgidus