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Literature summary extracted from
- Purification and characterization of human placental aromatase cytochrome P-450 (1987), J. Biol. Chem., 262, 4413-20.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.14 | 0.00006 | - |
androst-4-ene-3,17-dione | pH 7.2, 37°C | Homo sapiens |  |
| 1.14.14.14 | 0.00021 | - |
testosterone | pH 7.2, 37°C | Homo sapiens | |
| 1.14.14.14 | 0.058 | - |
16alpha-hydroxytestosterone | pH 7.2, 37°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.14 | microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.14 | 55000 | - |
x * 55000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.14 | androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins | Homo sapiens | overall reaction | estrone + formate + 4 H2O + 3 oxidized flavoproteins | - |
? | |
| 1.14.14.14 | testosterone + 3 O2 + 3 reduced flavoproteins | Homo sapiens | overall reaction | 17beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.14 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.14 | from microsome. Full enzymatic activity can be reconstituted with rabbit liver microsomal cytochrome P-450 reductase and Nonidet P-40 | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.14 | placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.14 | 57 | - |
pH 7.2, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.14 | 16alpha-hydroxytestosterone + 3 O2 + 3 reduced flavoprotein | overall reaction | Homo sapiens | (16alpha,17beta)-estra-1(10),2,4-triene-3,16,17-triol + formate + 4 H2O + 3 oxidized flavoprotein | - |
? | |
| 1.14.14.14 | androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins | overall reaction | Homo sapiens | estrone + formate + 4 H2O + 3 oxidized flavoproteins | - |
? | |
| 1.14.14.14 | testosterone + 3 O2 + 3 reduced flavoproteins | overall reaction | Homo sapiens | 17beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.14 | ? | x * 55000, SDS-PAGE | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.14 | cytochrome P450 | specific content of 11.5 nmol of P450/mg of protein. Enzyme displays spectroscopic properties typical of the ferric and ferrous forms of cytochrome P-450 | Homo sapiens |
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