EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.10 | expressed in Sulfolobus acidocaldarius. Attempts to obtain soluble enzyme from Escherichia coli strains are unsuccessful | Sulfolobus acidocaldarius |
3.2.1.20 | expressed in Sulfolobus acidocaldarius. Attempts to obtain a soluble enzyme from Escherichia coli strains are unsuccessful | Sulfolobus acidocaldarius |
3.2.1.84 | expressed in Sulfolobus acidocaldarius. Attempts to obtain soluble enzyme from Escherichia coli strains are unsuccessful | Sulfolobus acidocaldarius |
3.2.1.216 | expressed in Sulfolobus acidocaldarius. Attempts to obtain soluble enzyme from Escherichia coli strains are unsuccessful | Sulfolobus acidocaldarius |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | Co2+ | 1 mM, pH 5.0, 95°C, 24% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
3.2.1.20 | Cu2+ | 1 mM, pH 5.0, 95°C, 91% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
3.2.1.20 | EDTA | 1 mM, pH 5.0, 95°C, 11% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
3.2.1.20 | Fe2+ | 1 mM, pH 5.0, 95°C, 39% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
3.2.1.20 | SDS | 1%, pH 5.0, 95°C, complete inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
3.2.1.20 | Urea | 1 mM, pH 5.0, 95°C, 26% inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius | |
3.2.1.20 | Zn2+ | 1 mM, pH 5.0, 95°C, complete inhibition of hydrolysis of 4-nitrophenyl alpha-D-glucopyranoside | Sulfolobus acidocaldarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 0.3 | - |
maltotriose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 0.3 | - |
maltotetraose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 0.32 | - |
maltopentaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 0.52 | - |
maltohexaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 0.8 | - |
maltose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 0.87 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.0, 95°C | Sulfolobus acidocaldarius |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.10 | intracellular | - |
Sulfolobus acidocaldarius | 5622 | - |
3.2.1.20 | intracellular | - |
Sulfolobus acidocaldarius | 5622 | - |
3.2.1.84 | intracellular | - |
Sulfolobus acidocaldarius | 5622 | - |
3.2.1.216 | intracellular | - |
Sulfolobus acidocaldarius | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | Mn2+ | 1 mM, pH 5.0, 95°C, 1.7fold activation of hydrolysis of 4-nitrophenyl alpha-glucopyranoside | Sulfolobus acidocaldarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 72000 | - |
12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.10 | 73000 | - |
12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.10 | 914000 | - |
gel filtration | Sulfolobus acidocaldarius |
3.2.1.20 | 72000 | - |
12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.20 | 73000 | - |
12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.20 | 914000 | - |
gel filtration | Sulfolobus acidocaldarius |
3.2.1.84 | 72000 | - |
12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.84 | 73000 | - |
12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.84 | 914000 | - |
gel filtration | Sulfolobus acidocaldarius |
3.2.1.216 | 72000 | - |
12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.216 | 73000 | - |
12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.216 | 914000 | - |
gel filtration | Sulfolobus acidocaldarius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | maltose + H2O | Sulfolobus acidocaldarius | the enzyme is involved in maltose and starch metabolism | alpha-D-glucose + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.10 | Sulfolobus acidocaldarius | Q4J9M3 | - |
- |
3.2.1.10 | Sulfolobus acidocaldarius DSM 639 | Q4J9M3 | - |
- |
3.2.1.20 | Sulfolobus acidocaldarius | Q4J9M3 | - |
- |
3.2.1.20 | Sulfolobus acidocaldarius DSM 639 | Q4J9M3 | - |
- |
3.2.1.84 | Sulfolobus acidocaldarius | Q4J9M3 | - |
- |
3.2.1.84 | Sulfolobus acidocaldarius DSM 639 | Q4J9M3 | - |
- |
3.2.1.216 | Sulfolobus acidocaldarius | Q4J9M3 | - |
- |
3.2.1.216 | Sulfolobus acidocaldarius DSM 639 | Q4J9M3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.10 | - |
Sulfolobus acidocaldarius |
3.2.1.20 | - |
Sulfolobus acidocaldarius |
3.2.1.84 | - |
Sulfolobus acidocaldarius |
3.2.1.216 | - |
Sulfolobus acidocaldarius |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.10 | culture condition:maltose-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.10 | culture condition:starch-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for starch as a substrate in cell extracts is 10fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.20 | culture condition:maltose-grown cell | the transcription level of malA is increased 3-fold upon the addition of maltose to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11-fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.20 | culture condition:starch-grown cell | the transcription level of malA is increased 3-fold upon the addition of starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 10-fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, respectively, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.84 | culture condition:maltose-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.84 | culture condition:starch-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 10fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.216 | culture condition:maltose-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars | Sulfolobus acidocaldarius | - |
3.2.1.216 | culture condition:starch-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 10fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, than during growth on other sugars | Sulfolobus acidocaldarius | - |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.10 | 4°C, stored for at least 6 months without any loss of activity | Sulfolobus acidocaldarius |
3.2.1.20 | 4°C, stored for at least 6 months without any loss of activity | Sulfolobus acidocaldarius |
3.2.1.84 | 4°C, stored for at least 6 months without any loss of activity | Sulfolobus acidocaldarius |
3.2.1.216 | 4°C, stored for at least 6 months without any loss of activity | Sulfolobus acidocaldarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.10 | isomaltose + H2O | i.e. alpha-D-Glc-(1->6)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.10 | isomaltose + H2O | i.e. alpha-D-Glc-(1->6)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius DSM 639 | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.10 | panose + H2O | alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius | maltose + alpha-D-glucose | - |
? | |
3.2.1.10 | panose + H2O | alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius DSM 639 | maltose + alpha-D-glucose | - |
? | |
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Sulfolobus acidocaldarius | 4-nitrophenol + alpha-D-glucose | - |
? | |
3.2.1.20 | isomaltose + H2O | i.e. alpha-D-Glc-(1->6)-D-Glc | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | kojibiose + H2O | i.e. alpha-D-Glc-(1->2)-D-Glc | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | kojibiose + H2O | i.e. alpha-D-Glc-(1->2)-D-Glc | Sulfolobus acidocaldarius DSM 639 | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltohexaose + H2O | - |
Sulfolobus acidocaldarius | maltopentaose + D-glucose | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Sulfolobus acidocaldarius | alpha-D-glucose + ? | - |
? | |
3.2.1.20 | maltose + H2O | - |
Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | the enzyme is involved in maltose and starch metabolism | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltotetraose + H2O | - |
Sulfolobus acidocaldarius | alpha-D-glucose | - |
? | |
3.2.1.20 | maltotriose + H2O | - |
Sulfolobus acidocaldarius | maltose + alpha-D-glucose | - |
? | |
3.2.1.20 | additional information | no activity towards cellobiose, starch and glycogen | Sulfolobus acidocaldarius | ? | - |
? | |
3.2.1.20 | additional information | no activity towards cellobiose, starch and glycogen | Sulfolobus acidocaldarius DSM 639 | ? | - |
? | |
3.2.1.20 | nigerose + H2O | i.e. alpha-D-Glc-(1->3)-D-Glc | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | panose + H2O | alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc | Sulfolobus acidocaldarius | maltose + alpha-D-glucose | - |
? | |
3.2.1.84 | nigerose + H2O | i.e. alpha-D-Glc-(1->3)-D-Glc. The multifunctional enzyme also catalyzes the hydrolysis of terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,6-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.10 hydrolase) | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.84 | nigerose + H2O | i.e. alpha-D-Glc-(1->3)-D-Glc. The multifunctional enzyme also catalyzes the hydrolysis of terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,6-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.10 hydrolase) | Sulfolobus acidocaldarius DSM 639 | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.216 | kojibiose + H2O | i.e. alpha-D-Glc-(1->2)-D-Glc. Multifunctional enzyme that catalyzes also the hydrolysis of terminal alpha-1,4-linked, alpha-1,3-linked and alpha-1,6-linked glucose residues (EC 3.2.1.20/EC 3.2.1.84/EC 3.2.1.10) | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.10 | dodecamer | 12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.10 | dodecamer | 12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.20 | dodecamer | 12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.20 | dodecamer | 12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.84 | dodecamer | 12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.84 | dodecamer | 12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
3.2.1.216 | dodecamer | 12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
3.2.1.216 | dodecamer | 12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.10 | MalA | - |
Sulfolobus acidocaldarius |
3.2.1.10 | Saci1160 | locus name | Sulfolobus acidocaldarius |
3.2.1.20 | MalA | - |
Sulfolobus acidocaldarius |
3.2.1.20 | Saci1160 | locus name | Sulfolobus acidocaldarius |
3.2.1.84 | MalA | - |
Sulfolobus acidocaldarius |
3.2.1.84 | Saci1160 | locus name | Sulfolobus acidocaldarius |
3.2.1.216 | MalA | - |
Sulfolobus acidocaldarius |
3.2.1.216 | Saci1160 | locus name | Sulfolobus acidocaldarius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 95 | 100 | - |
Sulfolobus acidocaldarius |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 80 | 110 | 80°C: about 60% of maximal activity, 110°C: about 80% of maximal activity | Sulfolobus acidocaldarius |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.10 | 95 | - |
half-life: 33.8 h | Sulfolobus acidocaldarius |
3.2.1.10 | 100 | - |
half-life: 10.6 h | Sulfolobus acidocaldarius |
3.2.1.10 | 105 | - |
half-life: 1.8 h | Sulfolobus acidocaldarius |
3.2.1.20 | 95 | - |
half-life: 33.8 h | Sulfolobus acidocaldarius |
3.2.1.20 | 100 | - |
half-life: 10.6 h | Sulfolobus acidocaldarius |
3.2.1.20 | 105 | - |
half-life: 1.8 h | Sulfolobus acidocaldarius |
3.2.1.84 | 95 | - |
half-life: 33.8 h | Sulfolobus acidocaldarius |
3.2.1.84 | 100 | - |
half-life: 10.6 h | Sulfolobus acidocaldarius |
3.2.1.84 | 105 | - |
half-life: 1.8 h | Sulfolobus acidocaldarius |
3.2.1.216 | 95 | - |
half-life: 33.8 h | Sulfolobus acidocaldarius |
3.2.1.216 | 100 | - |
half-life: 10.6 h | Sulfolobus acidocaldarius |
3.2.1.216 | 105 | - |
half-life: 1.8 h | Sulfolobus acidocaldarius |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 16.7 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 372 | - |
maltohexaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 527 | - |
maltotetraose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 550 | - |
maltotriose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 611 | - |
maltopentaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 1278 | - |
maltose | pH 5.0, 95°C | Sulfolobus acidocaldarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 5 | - |
- |
Sulfolobus acidocaldarius |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4 | 7 | pH 4.0: about 35% of maximal activity, pH 7.0: about 40% of maximal activity | Sulfolobus acidocaldarius |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.10 | Sulfolobus acidocaldarius | calculated from sequence | - |
5.9 |
3.2.1.20 | Sulfolobus acidocaldarius | calculated from sequence | - |
5.9 |
3.2.1.84 | Sulfolobus acidocaldarius | calculated from sequence | - |
5.9 |
3.2.1.216 | Sulfolobus acidocaldarius | calculated from sequence | - |
5.9 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.2.1.10 | Sulfolobus acidocaldarius | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium | up |
3.2.1.20 | Sulfolobus acidocaldarius | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium | up |
3.2.1.84 | Sulfolobus acidocaldarius | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium | up |
3.2.1.216 | Sulfolobus acidocaldarius | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.20 | physiological function | the enzyme is involved in maltose and starch metabolism | Sulfolobus acidocaldarius |
3.2.1.216 | physiological function | the enzyme is involved in maltose and starch metabolism | Sulfolobus acidocaldarius |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 19.2 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 716 | - |
maltohexaose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 1597 | - |
maltose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 1759 | - |
maltotetraose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 1833 | - |
maltotriose | pH 5.0, 95°C | Sulfolobus acidocaldarius | |
3.2.1.20 | 1910 | - |
maltopentaose | pH 5.0, 95°C | Sulfolobus acidocaldarius |