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Literature summary extracted from

  • Giffin, M.M.; Modesti, L.; Raab, R.W.; Wayne, L.G.; Sohaskey, C.D.
    ald of Mycobacterium tuberculosis encodes both the alanine dehydrogenase and the putative glycine dehydrogenase (2012), J. Bacteriol., 194, 1045-1054.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566 Mycobacterium tuberculosis
1.4.1.10 gene ald, quantitative real-time PCR expression analysis, expression in Escherichia coli strain ER2566 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.1 additional information generation of ald knockout strain RVW7 from wild-type strain H37Rv Mycobacterium tuberculosis
1.4.1.10 additional information generation of ald knockout strain RVW7 from wild-type strain H37Rv Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 additional information
-
 additional information kinetics of ald and glyoxylate dehydrogenae activities, overview Mycobacterium tuberculosis
1.4.1.1 0.0384
-
 NADH pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
1.4.1.1 0.1328
-
 NAD+ pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
1.4.1.1 2.3
-
 NH3 pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
1.4.1.1 2.8
-
 pyruvate pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
1.4.1.1 4.3
-
 L-alanine pH 6.4, 22°C, recombinant His6-tagged enzyme Mycobacterium tuberculosis
1.4.1.10 5.5
-
 glyoxylate pH 8.5, 22°C, recombinant enzyme Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.1 cytosol minor fraction Mycobacterium tuberculosis 5829
-
1.4.1.1 membrane major fraction Mycobacterium tuberculosis 16020
-
1.4.1.1 additional information no enzyme detected in the extracellular medium Mycobacterium tuberculosis
-
-
1.4.1.10 cytosol minor fraction Mycobacterium tuberculosis 5829
-
1.4.1.10 membrane major fraction Mycobacterium tuberculosis 16020
-
1.4.1.10 additional information no enzyme detected in the extracellular medium Mycobacterium tuberculosis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 L-alanine + H2O + NAD+ Mycobacterium tuberculosis
-
 pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 L-alanine + H2O + NAD+ Mycobacterium tuberculosis H37Rv
-
 pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 additional information Mycobacterium tuberculosis enzyme Ald has both pyruvate and glyoxylate aminating activities ?
-
 ?
1.4.1.1 additional information Mycobacterium tuberculosis H37Rv enzyme Ald has both pyruvate and glyoxylate aminating activities ?
-
 ?
1.4.1.1 pyruvate + NH3 + NADH + H+ Mycobacterium tuberculosis
-
 L-alanine + H2O + NAD+
-
 r
1.4.1.1 pyruvate + NH3 + NADH + H+ Mycobacterium tuberculosis H37Rv
-
 L-alanine + H2O + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Mycobacterium tuberculosis
-
 gene ald
-
1.4.1.1 Mycobacterium tuberculosis H37Rv
-
 gene ald
-
1.4.1.10 Mycobacterium tuberculosis
-
 gene ald
-
1.4.1.10 Mycobacterium tuberculosis H37Rv
-
 gene ald
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration Mycobacterium tuberculosis
1.4.1.10 recombinant His-tagged enzyme from Escherichia coli strain ER2566 by nickel affinity chromatography, native enzyme by anion exchange and hydrophobic interaction chromatography, followed by ultrafiltration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 additional information
-
 activities in different strains, overview Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-alanine + H2O + NAD+
-
 Mycobacterium tuberculosis pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 L-alanine + H2O + NAD+
-
 Mycobacterium tuberculosis H37Rv pyruvate + NH3 + NADH + H+
-
 r
1.4.1.1 additional information enzyme Ald has both pyruvate and glyoxylate aminating activities Mycobacterium tuberculosis ?
-
 ?
1.4.1.1 additional information enzyme Ald has both pyruvate and glyoxylate aminating activities Mycobacterium tuberculosis H37Rv ?
-
 ?
1.4.1.1 pyruvate + NH3 + NADH + H+
-
 Mycobacterium tuberculosis L-alanine + H2O + NAD+
-
 r
1.4.1.1 pyruvate + NH3 + NADH + H+
-
 Mycobacterium tuberculosis H37Rv L-alanine + H2O + NAD+
-
 r
1.4.1.10 glyoxylate + NH3 + NADH + H+
-
 Mycobacterium tuberculosis glycine + H2O + NAD+
-
 ir
1.4.1.10 glyoxylate + NH3 + NADH + H+
-
 Mycobacterium tuberculosis H37Rv glycine + H2O + NAD+
-
 ir
1.4.1.10 additional information the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity Mycobacterium tuberculosis ?
-
 ?
1.4.1.10 additional information the putative glycine dehydrogenase of Mycobacterium tuberculosis catalyzes the reductive amination of glyoxylate to glycine but not the reverse reaction. It exhbits both pyruvate and glyoxylate aminating activitiesand also shows alanine dehydrogenase activity, EC 1.4.1.1, to a greater extent than glyoxylate aminating activity Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 ALD
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 22
-
 assay at room temperature Mycobacterium tuberculosis
1.4.1.10 22
-
 assay at room temperature Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 9
-
 ALD activity Mycobacterium tuberculosis
1.4.1.10 8.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Mycobacterium tuberculosis
1.4.1.1 NADH
-
 Mycobacterium tuberculosis
1.4.1.10 NADH
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
1.4.1.1 Mycobacterium tuberculosis transcription of ald is induced when alanine is the sole nitrogen source up
1.4.1.10 Mycobacterium tuberculosis transcription of ald is induced when alanine is the sole nitrogen source up

General Information

EC Number General Information Comment Organism
1.4.1.1 malfunction an ald knockout strain grows without alanine or glycine and is able to utilize glycine but not alanine as a nitrogen source Mycobacterium tuberculosis
1.4.1.1 physiological function enzyme Ald may have several functions, including ammonium incorporation and alanine breakdown. Ald plays an essential role in the utilization of alanine but not of glycine. Ald is not essential for the breakdown of glycine Mycobacterium tuberculosis
1.4.1.10 physiological function the enzyme plays an essential role in the utilization of alanine, but not of glycine, as a nitrogen source Mycobacterium tuberculosis