Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chuakrut, S.; Arai, H.; Ishii, M.; Igarashi, Y.
    Characterization of a bifunctional archaeal acyl coenzyme A carboxylase (2003), J. Bacteriol., 185, 938-947.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 biotin biotin-dependent enzyme Acidianus brierleyi
6.4.1.2 palmitoyl-CoA slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) Acidianus brierleyi
6.4.1.3 biotin biotin-dependent enzyme Acidianus brierleyi
6.4.1.3 palmitoyl-CoA slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) Acidianus brierleyi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.2
-
 Acidianus brierleyi
6.4.1.3
-
 Acidianus brierleyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ATP above 2.0 mM Acidianus brierleyi
6.4.1.2 citrate 3 mM, more than 50% inhibition Acidianus brierleyi
6.4.1.2 CoA 1 mM, 11-13% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
6.4.1.2 DTT 5-10 mM, 12% inhibition Acidianus brierleyi
6.4.1.2 K+
-
 Acidianus brierleyi
6.4.1.2 malonyl-CoA 0.5 mM, 50-57% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
6.4.1.2 methylmalonyl-CoA 0.5 mM, 79% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
6.4.1.2 Mg2+ above 4.0 mM Acidianus brierleyi
6.4.1.2 Mn2+ above 2.0 mM Acidianus brierleyi
6.4.1.2 Na+
-
 Acidianus brierleyi
6.4.1.2 succinyl-CoA 0.5 mM, 84% inhibition of acetyl-CoA carboxylase Acidianus brierleyi
6.4.1.2 sulfate 25 mM, more than 50% inhibition Acidianus brierleyi
6.4.1.3 ATP above 2.0 mM Acidianus brierleyi
6.4.1.3 citrate 3 mM, more than 50% inhibition Acidianus brierleyi
6.4.1.3 CoA 1 mM, 60% inhibition of propionyl-CoA carboxylase Acidianus brierleyi
6.4.1.3 DTT 5-10 mM, 12% inhibition Acidianus brierleyi
6.4.1.3 K+
-
 Acidianus brierleyi
6.4.1.3 malonyl-CoA 0.5 mM, 60% inhibition of propionyl-CoA carboxylase Acidianus brierleyi
6.4.1.3 methylmalonyl-CoA 0.5 mM, 60% inhibition of propionyl-CoA carboxylase Acidianus brierleyi
6.4.1.3 Mg2+ above 4.0 mM Acidianus brierleyi
6.4.1.3 Mn2+ above 2.0 mM Acidianus brierleyi
6.4.1.3 Na+
-
 Acidianus brierleyi
6.4.1.3 succinyl-CoA 0.5 mM, 57% inhibition of propionyl-CoA carboxylase Acidianus brierleyi
6.4.1.3 sulfate 25 mM, more than 50% inhibition Acidianus brierleyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.17
-
 acetyl-CoA pH 7.5, 70°C Acidianus brierleyi
6.4.1.3 0.1
-
 propanoyl-CoA pH 7.5, 70°C Acidianus brierleyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Co2+ 26% of the maximum activity is observed when Co2+ (10 to 15 mM) is used as a divalent cation Acidianus brierleyi
6.4.1.2 Mg2+ maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM Acidianus brierleyi
6.4.1.2 Mn2+ 1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion Acidianus brierleyi
6.4.1.3 Co2+ 26% of the maximum activity is observed when Co2+(10 to 15 mM) is used as a divalent cation Acidianus brierleyi
6.4.1.3 Mg2+ maximum acetyl-CoA carboxylase activity is obtained at 4.0 mM Acidianus brierleyi
6.4.1.3 Mn2+ 1.0 mM Mn2+ gives 82% of the maximum activity obtained with the Mg2+ ion Acidianus brierleyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 20000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.2 59000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.2 62000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.2 540000
-
-
 Acidianus brierleyi
6.4.1.3 20000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.3 59000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.3 62000
-
 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.3 540000
-
-
 Acidianus brierleyi

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Acidianus brierleyi Q877I4 and Q877I3 and Q877I5 Q877I4: biotin carboxyl carrier protein of acetyl-CoA carboxylase, Q877I3: carboxyltransferase subunit of acetyl-CoA carboxylase, Q877I5: biotin carboxylase subunit of acetyl-CoA carboxylase
-
6.4.1.3 Acidianus brierleyi Q877I4 and Q877I3 and Q877I5 Q877I4: biotin carboxyl carrier protein of acetyl-CoA carboxylase, Q877I3: carboxyltransferase subunit of acetyl-CoA carboxylase, Q877I5: biotin carboxylase subunit of acetyl-CoA carboxylase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Acidianus brierleyi
6.4.1.3
-
 Acidianus brierleyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 14.5
-
 pH 7.5, 70°C, acetyl-CoA carboxylase activity Acidianus brierleyi
6.4.1.3 11.9
-
 pH 7.5, 70°C, propionyl-CoA carboxylase activity Acidianus brierleyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- the bifunctional enzyme also catalyzes the carboxylation of propionyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM) Acidianus brierleyi ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3- n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA Acidianus brierleyi ?
-
 ?
6.4.1.2 additional information neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme Acidianus brierleyi ?
-
 ?
6.4.1.3 ATP + acetyl-CoA + HCO3- n-butyryl-CoA (0.5 mM) is carboxylated at 4.6% compared to carboxylation of acetyl-CoA or propionyl-CoA Acidianus brierleyi ?
-
 ?
6.4.1.3 ATP + propanoyl-CoA + HCO3- the bifunctional enzyme also catalyzes the carboxylation of acetyl-CoA. Vmax/Km for carboxylation of propanoyl-CoA is 1.6fold higher than Vmax/Km for carboxylation of acetyl-CoA. Quite specific for ATP. No activity with ADP, AMP, GTP, CTP, UTP, or dTTP (0.2 mM) Acidianus brierleyi ADP + phosphate + (S)-methylmalonyl-CoA
-
 ?
6.4.1.3 additional information neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme Acidianus brierleyi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 dodecamer 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi
6.4.1.3 dodecamer 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE Acidianus brierleyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.2 65
-
-
 Acidianus brierleyi
6.4.1.2 70
-
 assay at Acidianus brierleyi
6.4.1.3 65
-
-
 Acidianus brierleyi
6.4.1.3 70
-
 assay at Acidianus brierleyi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.4.1.2 50 75 50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity Acidianus brierleyi
6.4.1.3 50 75 50°C: about 60% of maximal activity, 75°C: about 60% of maximal activity Acidianus brierleyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.2 50
-
 2 h, 40% loss of activity Acidianus brierleyi
6.4.1.2 60
-
 30 min, 50% loss of activity Acidianus brierleyi
6.4.1.2 70
-
 30 min, 90% loss of activity Acidianus brierleyi
6.4.1.2 80
-
 30 min, 95% loss of activity Acidianus brierleyi
6.4.1.2 90
-
 10 min, complete loss of activity Acidianus brierleyi
6.4.1.3 50
-
 2 h, 40% loss of activity Acidianus brierleyi
6.4.1.3 60
-
 30 min, 50% loss of activity Acidianus brierleyi
6.4.1.3 70
-
 30 min, 90% loss of activity Acidianus brierleyi
6.4.1.3 80
-
 30 min, 95% loss of activity Acidianus brierleyi
6.4.1.3 90
-
 10 min, complete loss of activity Acidianus brierleyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 6.4 6.9 at 70°C Acidianus brierleyi
6.4.1.2 7.5
-
 assay at Acidianus brierleyi
6.4.1.3 6.4 6.9 at 70°C Acidianus brierleyi
6.4.1.3 7.5
-
 assay at Acidianus brierleyi