EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.147 | 0.2 | - |
formaldehyde | 30°C, pH 8.0 | Methylorubrum extorquens | |
4.2.1.147 | 1 | - |
5,6,7,8-tetrahydromethanopterin | 30°C, pH 8.0 | Methylorubrum extorquens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.147 | cytoplasm | one of the major proteins in the cytoplasm | Methylorubrum extorquens | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.147 | Mg2+ | stimulates activity, Ka: 4 mM | Methylorubrum extorquens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.147 | 18000 | - |
3 * 18000, SDS-PAGE | Methylorubrum extorquens |
4.2.1.147 | 60000 | - |
gel filtration | Methylorubrum extorquens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.147 | 5,6,7,8-tetrahydromethanopterin + formaldehyde | Methylorubrum extorquens | - |
5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
4.2.1.147 | 5,6,7,8-tetrahydromethanopterin + formaldehyde | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
5,10-methylenetetrahydromethanopterin + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.147 | Methylorubrum extorquens | Q9FA38 | - |
- |
4.2.1.147 | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | Q9FA38 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.147 | - |
Methylorubrum extorquens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.147 | 20.2 | - |
30°C, pH 8.0 | Methylorubrum extorquens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.147 | 5,6,7,8-tetrahydromethanopterin + formaldehyde | - |
Methylorubrum extorquens | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
4.2.1.147 | 5,6,7,8-tetrahydromethanopterin + formaldehyde | the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction | Methylorubrum extorquens | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
4.2.1.147 | 5,6,7,8-tetrahydromethanopterin + formaldehyde | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
4.2.1.147 | 5,6,7,8-tetrahydromethanopterin + formaldehyde | the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,10-methylenetetrahydromethanopterin + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.147 | homotrimer | 3 * 18000, SDS-PAGE | Methylorubrum extorquens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.147 | formaldehyde-activating enzyme | - |
Methylorubrum extorquens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.147 | 30 | - |
assay at | Methylorubrum extorquens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.147 | 8 | - |
assay at | Methylorubrum extorquens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.147 | malfunction | mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde | Methylorubrum extorquens |
4.2.1.147 | physiological function | the enzyme is required for growth on methanol | Methylorubrum extorquens |