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Literature summary extracted from

  • Vorholt, J.A.; Marx, C.J.; Lidstrom, M.E.; Thauer, R.K.
    Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol (2000), J. Bacteriol., 182, 6645-6650.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.147 0.2
-
 formaldehyde 30°C, pH 8.0 Methylorubrum extorquens
4.2.1.147 1
-
 5,6,7,8-tetrahydromethanopterin 30°C, pH 8.0 Methylorubrum extorquens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.147 cytoplasm one of the major proteins in the cytoplasm Methylorubrum extorquens 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.147 Mg2+ stimulates activity, Ka: 4 mM Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.147 18000
-
 3 * 18000, SDS-PAGE Methylorubrum extorquens
4.2.1.147 60000
-
 gel filtration Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde Methylorubrum extorquens
-
 5,10-methylenetetrahydromethanopterin + H2O
-
 ?
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
 5,10-methylenetetrahydromethanopterin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.147 Methylorubrum extorquens Q9FA38
-
-
4.2.1.147 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 Q9FA38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.147
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.147 20.2
-
 30°C, pH 8.0 Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde
-
 Methylorubrum extorquens 5,10-methylenetetrahydromethanopterin + H2O
-
 ?
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction Methylorubrum extorquens 5,10-methylenetetrahydromethanopterin + H2O
-
 ?
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde
-
 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 5,10-methylenetetrahydromethanopterin + H2O
-
 ?
4.2.1.147 5,6,7,8-tetrahydromethanopterin + formaldehyde the reaction also proceeds spontaneously, but at a lower rate than that of the enzyme-catalyzed reaction Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 5,10-methylenetetrahydromethanopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.147 homotrimer 3 * 18000, SDS-PAGE Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.147 formaldehyde-activating enzyme
-
 Methylorubrum extorquens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.147 30
-
 assay at Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.147 8
-
 assay at Methylorubrum extorquens

General Information

EC Number General Information Comment Organism
4.2.1.147 malfunction mutants of Methylobacterium extorquens AM1 defective in Fae are able to grow on succinate but not on methanol and are much more sensitive toward methanol and formaldehyde Methylorubrum extorquens
4.2.1.147 physiological function the enzyme is required for growth on methanol Methylorubrum extorquens