EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.B2 | expressed in Escherichia coli | Streptomyces sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.B2 | H420F | inactive | Streptomyces sp. |
1.11.1.B2 | S427H | the mutant shows nearly wild type activity at approximately 70% in the monochlorodimedone assay but is unable to catalyze the conversion of 3 into 4 or 5. Instead, the mutant exclusively catalyzes the formation of 3-based bromohydrins | Streptomyces sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.B2 | Vanadium | dependent on | Streptomyces sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.B2 | Streptomyces sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.B2 | - |
Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.B2 | 3-chloro-2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxy-6-methyl-3-(3-methylbut-2-en-1-yl)-2,3-dihydronaphthalene-1,4-dione + bromide + H2O2 | - |
Streptomyces sp. | (3R)-3-bromo-4a-chloro-10a-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-6,8-dihydroxy-2,2,7-trimethyl-3,4,4a,10a-tetrahydro-2H-benzo[g]chromene-5,10-dione + H+ | - |
? | |
1.11.1.B2 | 3-chloro-2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxy-6-methyl-3-(3-methylbut-2-en-1-yl)-2,3-dihydronaphthalene-1,4-dione + chloride + H2O2 | - |
Streptomyces sp. | (3R)-3,4a-dichloro-10a-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-6,8-dihydroxy-2,2,7-trimethyl-3,4,4a,10a-tetrahydro-2H-benzo[g]chromene-5,10-dione + H+ | - |
? | |
1.11.1.B2 | monochlorodimedone + chloride + H2O2 | - |
Streptomyces sp. | dichlorodimedone + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.B2 | NapH1 | - |
Streptomyces sp. |
1.11.1.B2 | vanadium-dependent chloroperoxidase | - |
Streptomyces sp. |
1.11.1.B2 | vanadium-dependent haloperoxidase | - |
Streptomyces sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.11.1.B2 | metabolism | the enzyme catalyzes a highly stereoselective chlorination-cyclization reaction in napyradiomycin antibiotic biosynthesis | Streptomyces sp. |