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Literature summary extracted from

  • Bernhardt, P.; Okino, T.; Winter, J.M.; Miyanaga, A.; Moore, B.S.
    A stereoselective vanadium-dependent chloroperoxidase in bacterial antibiotic biosynthesis (2011), J. Am. Chem. Soc., 133, 4268-4270.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.B2 expressed in Escherichia coli Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.B2 H420F inactive Streptomyces sp.
1.11.1.B2 S427H the mutant shows nearly wild type activity at approximately 70% in the monochlorodimedone assay but is unable to catalyze the conversion of 3 into 4 or 5. Instead, the mutant exclusively catalyzes the formation of 3-based bromohydrins Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.B2 Vanadium dependent on Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B2 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.B2
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B2 3-chloro-2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxy-6-methyl-3-(3-methylbut-2-en-1-yl)-2,3-dihydronaphthalene-1,4-dione + bromide + H2O2
-
 Streptomyces sp. (3R)-3-bromo-4a-chloro-10a-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-6,8-dihydroxy-2,2,7-trimethyl-3,4,4a,10a-tetrahydro-2H-benzo[g]chromene-5,10-dione + H+
-
 ?
1.11.1.B2 3-chloro-2-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,5,7-trihydroxy-6-methyl-3-(3-methylbut-2-en-1-yl)-2,3-dihydronaphthalene-1,4-dione + chloride + H2O2
-
 Streptomyces sp. (3R)-3,4a-dichloro-10a-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-6,8-dihydroxy-2,2,7-trimethyl-3,4,4a,10a-tetrahydro-2H-benzo[g]chromene-5,10-dione + H+
-
 ?
1.11.1.B2 monochlorodimedone + chloride + H2O2
-
 Streptomyces sp. dichlorodimedone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.B2 NapH1
-
 Streptomyces sp.
1.11.1.B2 vanadium-dependent chloroperoxidase
-
 Streptomyces sp.
1.11.1.B2 vanadium-dependent haloperoxidase
-
 Streptomyces sp.

General Information

EC Number General Information Comment Organism
1.11.1.B2 metabolism the enzyme catalyzes a highly stereoselective chlorination-cyclization reaction in napyradiomycin antibiotic biosynthesis Streptomyces sp.