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Literature summary extracted from

  • Zhang, R.; Xu, C.; Shen, Q.; Kasuga, T.; Wu, W.; Szewczyk, E.; Ma, D.; Fan, Z.
    Characterization of two cellobiose dehydrogenases and comparison of their contributions to total activity in Neurospora crassa (2013), Int. Biodeter. Biodegrad., 82, 24-32.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 expressed in Neurospora crassa strain 2489 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.00579
-
 cellobiose isoform CDH2, at pH 6.0 and 50°C Neurospora crassa
1.1.99.18 0.02572
-
 cellobiose isoform CDH1, at pH 6.0 and 50°C Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
-
 Neurospora crassa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 86000
-
 1 * 86000, mature peptides, calculated from amino acid sequence Neurospora crassa
1.1.99.18 95000
-
 1 * 95000, deglycosylated enzyme forms, SDS-PAGE Neurospora crassa
1.1.99.18 100000
-
 1 * 100000, isoform CDH1, SDS-PAGE Neurospora crassa
1.1.99.18 130000
-
 1 * 130000, isoform CDH2, SDS-PAGE Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Neurospora crassa
-
-
-
1.1.99.18 Neurospora crassa FGSC 2489
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.18 glycoprotein
-
 Neurospora crassa

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl S-200 gel filtration Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture condition:avicel-grown cell
-
 Neurospora crassa
-
1.1.99.18 culture condition:rice straw-grown cell
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 0.28
-
 isoform CDH2 from extracellular medium, at pH 6.0 and 50°C Neurospora crassa
1.1.99.18 1.19
-
 isoform CDH1 from extracellular medium, at pH 6.0 and 50°C Neurospora crassa
1.1.99.18 7.93
-
 isoform CDH2 after 28.32fold purification, at pH 6.0 and 50°C Neurospora crassa
1.1.99.18 8.89
-
 isoform CDH1 after 7.47fold purification, at pH 6.0 and 50°C Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa FGSC 2489 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.99.18 additional information isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value Neurospora crassa ?
-
 ?
1.1.99.18 additional information isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value Neurospora crassa FGSC 2489 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 monomer 1 * 100000, isoform CDH1, SDS-PAGE Neurospora crassa
1.1.99.18 monomer 1 * 130000, isoform CDH2, SDS-PAGE Neurospora crassa
1.1.99.18 monomer 1 * 86000, mature peptides, calculated from amino acid sequence Neurospora crassa
1.1.99.18 monomer 1 * 95000, deglycosylated enzyme forms, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 CDH1 isoform Neurospora crassa
1.1.99.18 Cdh2 isoform Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.18 50
-
-
 Neurospora crassa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.99.18 45 55 about 70% activity between 45 and 55°C Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.18 20 30 the enzymes are stable for 10 h at 20-30°C Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 12.7
-
 cellobiose isoform CDH2, at pH 6.0 and 50°C Neurospora crassa
1.1.99.18 13.5
-
 cellobiose isoform CDH1, at pH 6.0 and 50°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 6
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.18 5 7.5 about 50% activity between pH 5.0 and 7.5 Neurospora crassa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.18 3 8 enzyme activities decrease sharply when stored in buffers with pHs below 3.0 or above 8.0 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 flavin
-
 Neurospora crassa
1.1.99.18 heme
-
 Neurospora crassa

Expression

EC Number Organism Comment Expression
1.1.99.18 Neurospora crassa the enzyme is produced when avicel or rice straw is used as the carbon source up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 525
-
 cellobiose isoform CDH1, at pH 6.0 and 50°C Neurospora crassa
1.1.99.18 2193
-
 cellobiose isoform CDH2, at pH 6.0 and 50°C Neurospora crassa