Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pundir, C.; Chauhan, N.; Kumari, G.; Vandana, C.
    Immobilization of Arthrobacter sarcosine oxidase onto alkylamine and arylamine glass and its application in serum sarcosine determination (2011), Indian J. Biotechnol., 10, 219-223.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.1 additional information immobilization of the enzyme onto alkylamine and arylamine glass by crosslinking with glutaraldehyde for determination of serum sarcosine, method, overview. The enzyme shows a decrease in optimum pH and KM, and an increase in optimum temperature and time for linearity after immobilization Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 0.285
-
sarcosine immobilized enzyme on alkylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
1.5.3.1 1.379
-
sarcosine immobilized enzyme on arylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
1.5.3.1 50
-
sarcosine free enzyme, pH 7.8, 37°C Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2 Arthrobacter sp.
-
glycine + formaldehyde + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Arthrobacter sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.1 commercial preparation
-
Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.1 1.81
-
immobilized enzyme on alkylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
1.5.3.1 2.02
-
immobilized enzyme on arylamine glass beads, pH 7.8, 37°C Arthrobacter sp.
1.5.3.1 2.38
-
soluble enzyme, pH 7.8, 37°C Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2
-
Arthrobacter sp. glycine + formaldehyde + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.1 sarcosine: O2 oxidoreductase
-
Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.1 37
-
free enzyme Arthrobacter sp.
1.5.3.1 37 40 immobilized enzyme Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.1 7.5
-
immobilized enzyme Arthrobacter sp.
1.5.3.1 9
-
free enzyme Arthrobacter sp.