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Literature summary extracted from

  • Liers, C.; Ullrich, R.; Hofrichter, M.; Minibayeva, F.V.; Beckett, R.P.
    A heme peroxidase of the ascomyceteous lichen Leptogium saturninum oxidizes high-redox potential substrates (2011), Fungal Genet. Biol., 48, 1139-1145.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.7 cytosol
-
 Leptogium saturninum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 42000
-
 1 * 79000 or 2 * 42000, SDS-PAGE Leptogium saturninum
1.11.1.7 79000
-
 1 * 79000 or 2 * 42000, SDS-PAGE Leptogium saturninum
1.11.1.7 81000
-
 gel filtration Leptogium saturninum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Leptogium saturninum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 Q Sepharose column chromatography and Mono Q column chromatography Leptogium saturninum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 0.03
-
 with Azure B as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 0.1
-
 with veratryl alcohol as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 0.4
-
 with 4-nitrophenol as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 0.5
-
 crude extract, in sodium malonate or citrate buffer (50 mM, pH 4.5), with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) as substrate, temperature not specified in the publication Leptogium saturninum
1.11.1.7 0.8
-
 with 4-hydroxybenzonitrile as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 5.3
-
 with 4-hydroxybenzoic acid as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 7
-
 with Reactive Black 5 as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 17
-
 with NADH as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 20
-
 with Reactive Blue 5 as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 53
-
 with 2,6-dimethoxyphenol as substrate, in sodium malonate or citrate buffer (50 mM, pH 4.5), temperature not specified in the publication Leptogium saturninum
1.11.1.7 243.3
-
 after 486.6fold purification, in sodium malonate or citrate buffer (50 mM, pH 4.5), with reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) as substrate, temperature not specified in the publication Leptogium saturninum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 2,6-dimethoxyphenol + H2O2 lowest specific activity Leptogium saturninum ? + H2O
-
 ?
1.11.1.7 4-hydroxybenzoic acid + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 4-hydroxybenzonitrile + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 4-nitrophenol + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 adlerol + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 Azure B + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 additional information no activity with Mn2+ at pH 4.5 and with H2O2 at pH 3.0 Leptogium saturninum ?
-
 ?
1.11.1.7 NADH + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 Reactive Black 5 + H2O2
-
 Leptogium saturninum ?
-
 ?
1.11.1.7 Reactive Blue 5 + H2O2 highest specific activity Leptogium saturninum ?
-
 ?
1.11.1.7 reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Leptogium saturninum oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
 ?
1.11.1.7 veratryl alcohol + H2O2
-
 Leptogium saturninum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 monomer or dimer 1 * 79000 or 2 * 42000, SDS-PAGE Leptogium saturninum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 POX
-
 Leptogium saturninum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.7 2 4 the activity of the enzyme for ABTS ranges between 100% and 55% at pH 3.0-4.0 and for 2,6-dimethoxyphenol at pH 2.0-4.0 Leptogium saturninum

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
 Leptogium saturninum