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Literature summary extracted from

  • Sadre, R.; Gruber, J.; Frentzen, M.
    Characterization of homogentisate prenyltransferases involved in plastoquinone-9 and tocochromanol biosynthesis (2006), FEBS Lett., 580, 5357-5362.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.115 n-dodecyl beta-D-maltoside stimulation at low concentration, strong inhibition above 0.1 mM Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.115
-
 Arabidopsis thaliana
2.5.1.115
-
 Synechocystis sp.
2.5.1.117 expression in Escherichia coli Chlamydomonas reinhardtii
2.5.1.117 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.115 n-dodecyl beta-D-maltoside stimulation at low concentration, strong inhibition above 0.1 mM Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.115 0.001
-
 (2E,7R,11R)-3,7,11,15-tetramethylhexadec-2-en-1-yl trihydrogen diphosphate pH 7.5, 28°C Synechocystis sp.
2.5.1.115 0.006
-
 homogentisate pH 7.5, 28°C Synechocystis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.115 membrane
-
 Arabidopsis thaliana 16020
-
2.5.1.115 membrane
-
 Synechocystis sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.115 Co2+ 5-10fold less stimulation than Mg2+ Synechocystis sp.
2.5.1.115 Mg2+ best activator, optimum concentration 2 mM Synechocystis sp.
2.5.1.115 Mn2+ 5-10fold less stimulation than Mg2+ Synechocystis sp.
2.5.1.115 additional information divalent cation required Synechocystis sp.
2.5.1.117 Mg2+ required Chlamydomonas reinhardtii
2.5.1.117 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate Chlamydomonas reinhardtii the enzyme is involved in biosynthesis pathway of plastoquinol-9 diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2
-
 ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate Arabidopsis thaliana the enzyme is involved in biosynthesis pathway of plastoquinol-9 diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2
-
 ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate Chlamydomonas reinhardtii CC-1690 the enzyme is involved in biosynthesis pathway of plastoquinol-9 diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.115 Arabidopsis thaliana Q8VWJ1
-
-
2.5.1.115 Synechocystis sp. P73726
-
-
2.5.1.117 Arabidopsis thaliana Q9SF04
-
-
2.5.1.117 Chlamydomonas reinhardtii A1JHN0
-
-
2.5.1.117 Chlamydomonas reinhardtii CC-1690 A1JHN0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.115 geranylgeranyl diphosphate + homogentisate
-
 Arabidopsis thaliana diphosphate + 2-methyl-6-geranylgeranylbenzene-1,4-diol + CO2
-
 ?
2.5.1.115 geranylgeranyl diphosphate + homogentisate
-
 Synechocystis sp. diphosphate + 2-methyl-6-geranylgeranylbenzene-1,4-diol + CO2
-
 ?
2.5.1.115 additional information enzyme is more active with phythyl diphosphate than with geranylgeranyl diphosphate but inactive with solanesyl diphosphate or farnesyl diphosphate Arabidopsis thaliana ?
-
 ?
2.5.1.115 additional information enzyme is more active with phythyl diphosphate than with geranylgeranyl diphosphate but inactive with solanesyl diphosphate or farnesyl diphosphate Synechocystis sp. ?
-
 ?
2.5.1.115 phytyl diphosphate + homogentisate
-
 Arabidopsis thaliana diphosphate + 2-methyl-6-phytylbenzene-1,4-diol + CO2
-
 ?
2.5.1.115 phytyl diphosphate + homogentisate
-
 Synechocystis sp. diphosphate + 2-methyl-6-phytylbenzene-1,4-diol + CO2
-
 ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate the enzyme is involved in biosynthesis pathway of plastoquinol-9 Chlamydomonas reinhardtii diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2
-
 ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate the enzyme is involved in biosynthesis pathway of plastoquinol-9 Arabidopsis thaliana diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2
-
 ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate highest activities with solanesyl diphosphate, hardly active with phytyl diphosphat Arabidopsis thaliana diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol i.e. 2-methyl-6-solanesylbenzene-1,4-diol ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate highest activity with solanesyl diphosphate, hardly active with phytyl diphosphat Chlamydomonas reinhardtii diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol i.e. 2-methyl-6-solanesylbenzene-1,4-diol ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate the enzyme is involved in biosynthesis pathway of plastoquinol-9 Chlamydomonas reinhardtii CC-1690 diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2
-
 ?
2.5.1.117 all-trans-nonaprenyl diphosphate + homogentisate highest activity with solanesyl diphosphate, hardly active with phytyl diphosphat Chlamydomonas reinhardtii CC-1690 diphosphate + 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol + CO2 2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol i.e. 2-methyl-6-solanesylbenzene-1,4-diol ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.115 At2g18950
-
 Arabidopsis thaliana
2.5.1.115 HPT
-
 Arabidopsis thaliana
2.5.1.115 HPT
-
 Synechocystis sp.
2.5.1.115 slr1736 locus name Synechocystis sp.
2.5.1.115 VTE2-1
-
 Arabidopsis thaliana
2.5.1.117 At3g11950
-
 Arabidopsis thaliana
2.5.1.117 AtHST
-
 Arabidopsis thaliana
2.5.1.117 CrHST
-
 Chlamydomonas reinhardtii
2.5.1.117 HST
-
 Chlamydomonas reinhardtii
2.5.1.117 PDS2
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.117 28
-
 assay at Chlamydomonas reinhardtii
2.5.1.117 28
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.115 7.5
-
-
 Arabidopsis thaliana
2.5.1.115 7.5
-
-
 Synechocystis sp.
2.5.1.117 8.5
-
 assay at Chlamydomonas reinhardtii
2.5.1.117 8.5
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.5.1.117 physiological function the enzyme is involved in biosynthesis pathway of plastoquinol-9 Chlamydomonas reinhardtii
2.5.1.117 physiological function the enzyme is involved in biosynthesis pathway of plastoquinol-9. Overexpression of At3g11950 in Arabidopsis thaliana, however, suggests that the solanesyltransferase can affect tocopherol biosynthesis as well Arabidopsis thaliana