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Literature summary extracted from

  • Berk, H.; Thauer, R.K.
    F420H2:NADP oxidoreductase from Methanobacterium thermoautotrophicum: identification of the encoding gene via functional overexpression in Escherichia coli (1998), FEBS Lett., 438, 124-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.40 overexpression in Escherichia coli Methanothermobacter thermautotrophicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.40 hanging drop method, 4°C Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.40 0.05
-
 NADPH pH 6.0, temperature not specified in the publication Methanothermobacter thermautotrophicus
1.5.1.40 0.07
-
 NADP+ pH 8.0, temperature not specified in the publication Methanothermobacter thermautotrophicus
1.5.1.40 0.15
-
 reduced coenzyme F420 pH 8.0, temperature not specified in the publication Methanothermobacter thermautotrophicus
1.5.1.40 0.3
-
 oxidized coenzyme F420 pH 6.0, temperature not specified in the publication Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.40 23500
-
 4 * 23500, calculated from sequence Methanothermobacter thermautotrophicus
1.5.1.40 28000
-
 4 * 28000, SDS-PAGE Methanothermobacter thermautotrophicus
1.5.1.40 110000
-
 gel filtration Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.40 Methanothermobacter thermautotrophicus D9PVP5
-
-
1.5.1.40 Methanothermobacter thermautotrophicus DSM 2133 D9PVP5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.40
-
 Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
1.5.1.40 4°C, purified enzyme at a protein concentration of 20 mg/ml is completely stable when stored at 4°C in 50 mM Tricine/KOH pH 8.0 containing 0.2 M KCl Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.40 oxidized coenzyme F420 + NADPH + H+
-
 Methanothermobacter thermautotrophicus reduced coenzyme F420 + NADP+
-
 ?
1.5.1.40 oxidized coenzyme F420 + NADPH + H+
-
 Methanothermobacter thermautotrophicus DSM 2133 reduced coenzyme F420 + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.40 homotetramer 4 * 28000, SDS-PAGE Methanothermobacter thermautotrophicus
1.5.1.40 homotetramer 4 * 23500, calculated from sequence Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.5.1.40 F420H2:NADP oxidoreductase
-
 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.40 3.5
-
 reduction of oxidized coenzyme F420 with NADPH Methanothermobacter thermautotrophicus
1.5.1.40 8
-
 reduction of NADP+ with reduced F420H2 Methanothermobacter thermautotrophicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.40 Methanothermobacter thermautotrophicus calculated from sequence
-
 4.6