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Literature summary extracted from

  • Nagata, N.; Kusakari, Y.; Fukunishi, Y.; Inoue, T.; Urade, Y.
    Catalytic mechanism of the primary human prostaglandin F2alpha synthase, aldo-keto reductase 1B1 - prostaglandin D2 synthase activity in the absence of NADP(H) (2011), FEBS J., 278, 1288-1298.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.188 expression in Escherichia coli Mus musculus
1.1.1.188 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.188 D43E only trace activities in presence and absence of NADPH Homo sapiens
1.1.1.188 D43N only trace activities in presence and absence of NADPH Homo sapiens
1.1.1.188 H110A only trace activities in presence and absence of NADPH Homo sapiens
1.1.1.188 H110F only trace activities in presence and absence of NADPH Homo sapiens
1.1.1.188 K77L only trace activities in presence and absence of NADPH Homo sapiens
1.1.1.188 K77R only trace activities in presence and absence of NADPH Homo sapiens
1.1.1.188 Y48F 69% of wild-type activity in presence of NADPH, 138% of wild-type isomerization activity in absence of NADPH, 0.2% of wild-type activity reducing 4-nitrobenzaldehyde Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.188 0.029
-
 prostaglandin H2 pH 5.5, 37°C Homo sapiens
1.1.1.188 0.033
-
 prostaglandin H2 pH 5.0, 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.188 Homo sapiens P15121
-
-
1.1.1.188 Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.188 2.4
-
 pH 5.5, 37°C Homo sapiens
1.1.1.188 3.6
-
 pH 5.0, 37°C Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.188 4-nitrobenzaldehyde + NADPH + H+
-
 Mus musculus (4-nitrophenyl)methanol + NADP+
-
 ?
1.1.1.188 4-nitrobenzaldehyde + NADPH + H+
-
 Homo sapiens (4-nitrophenyl)methanol + NADP+
-
 ?
1.1.1.188 additional information in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.018 mM. This activity activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively Mus musculus ?
-
 ?
1.1.1.188 additional information in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.023 mM. This activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively Homo sapiens ?
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Mus musculus 9alpha,11beta-prostaglandin F2 + NADP+
-
 ?
1.1.1.188 prostaglandin H2 + NADPH + H+
-
 Homo sapiens 9alpha,11beta-prostaglandin F2 + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.188 5
-
-
 Mus musculus
1.1.1.188 5.5
-
-
 Homo sapiens