BRENDA - Enzyme Database show

Catalytic mechanism of the primary human prostaglandin F2alpha synthase, aldo-keto reductase 1B1 - prostaglandin D2 synthase activity in the absence of NADP(H)

Nagata, N.; Kusakari, Y.; Fukunishi, Y.; Inoue, T.; Urade, Y.; FEBS J. 278, 1288-1298 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Homo sapiens
1.1.1.188
expression in Escherichia coli
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
D43E
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
D43N
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
H110A
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
H110F
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
K77L
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
K77R
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
Y48F
69% of wild-type activity in presence of NADPH, 138% of wild-type isomerization activity in absence of NADPH, 0.2% of wild-type activity reducing 4-nitrobenzaldehyde
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.029
-
prostaglandin H2
pH 5.5, 37°C
Homo sapiens
1.1.1.188
0.033
-
prostaglandin H2
pH 5.0, 37°C
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Homo sapiens
P15121
-
-
1.1.1.188
Mus musculus
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.188
2.4
-
pH 5.5, 37°C
Homo sapiens
1.1.1.188
3.6
-
pH 5.0, 37°C
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
4-nitrobenzaldehyde + NADPH + H+
-
724961
Mus musculus
(4-nitrophenyl)methanol + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH + H+
-
724961
Homo sapiens
(4-nitrophenyl)methanol + NADP+
-
-
-
?
1.1.1.188
additional information
in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.018 mM. This activity activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively
724961
Mus musculus
?
-
-
-
-
1.1.1.188
additional information
in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.023 mM. This activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively
724961
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH + H+
-
724961
Mus musculus
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH + H+
-
724961
Homo sapiens
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
5
-
-
Mus musculus
1.1.1.188
5.5
-
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
expression in Escherichia coli
Homo sapiens
1.1.1.188
expression in Escherichia coli
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.188
D43E
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
D43N
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
H110A
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
H110F
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
K77L
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
K77R
only trace activities in presence and absence of NADPH
Homo sapiens
1.1.1.188
Y48F
69% of wild-type activity in presence of NADPH, 138% of wild-type isomerization activity in absence of NADPH, 0.2% of wild-type activity reducing 4-nitrobenzaldehyde
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.029
-
prostaglandin H2
pH 5.5, 37°C
Homo sapiens
1.1.1.188
0.033
-
prostaglandin H2
pH 5.0, 37°C
Mus musculus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.188
2.4
-
pH 5.5, 37°C
Homo sapiens
1.1.1.188
3.6
-
pH 5.0, 37°C
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
4-nitrobenzaldehyde + NADPH + H+
-
724961
Mus musculus
(4-nitrophenyl)methanol + NADP+
-
-
-
?
1.1.1.188
4-nitrobenzaldehyde + NADPH + H+
-
724961
Homo sapiens
(4-nitrophenyl)methanol + NADP+
-
-
-
?
1.1.1.188
additional information
in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.018 mM. This activity activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively
724961
Mus musculus
?
-
-
-
-
1.1.1.188
additional information
in the absence of NADPH or NADP+, enzyme catalyzes the isomerization of prostaglandin H2 to prostaglandin D2 with a Km value of 0.023 mM. This activity of AKR1B1 increases with increasing pH, with an optimum of pH 8.5. Residue His110 acts as a base in concert with Asp43 and Lys77 and as an acid to generate PGD2 and PGF2a in the absence of NADPH or NADP+ and in the presence of NADPH, respectively
724961
Homo sapiens
?
-
-
-
-
1.1.1.188
prostaglandin H2 + NADPH + H+
-
724961
Mus musculus
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH + H+
-
724961
Homo sapiens
9alpha,11beta-prostaglandin F2 + NADP+
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.188
5
-
-
Mus musculus
1.1.1.188
5.5
-
-
Homo sapiens