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Literature summary extracted from

  • Fang, T.Y.; Hung, X.G.; Shih, T.Y.; Tseng, W.C.
    Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092 (2004), Extremophiles, 8, 335-343 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.15 cloned from the genomic DNA of Sulfolobus solfataricus ATCC 35092 to an expression vector with a T7 lac promoter and then expressed in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.15 Cu2+ 4 mM, about 40% loss of activity Saccharolobus solfataricus
5.4.99.15 additional information Ca2+, K+, Mg2+, or Na+ have no activating or inhibiting effect on activity Saccharolobus solfataricus
5.4.99.15 Zn2+ 4 mM, about 60% loss of activity Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.99.15 additional information metal ions are not required for activity. Ca2+, K+, Mg2+, or Na+ have no activating or inhibiting effect on activity Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.15 86609
-
x * 86609, calculated from sequence Saccharolobus solfataricus
5.4.99.15 87000
-
x * 87000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.15 Saccharolobus solfataricus Q7LX98
-
-
5.4.99.15 Saccharolobus solfataricus P2 Q7LX98
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.15
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.15 19
-
pH 5.0, 75°C, substrate: maltohexaose Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 maltoheptaose preferred substrate, ratio of transglycosylation to hydrolysis is 100:0.2, recombinant enzyme Saccharolobus solfataricus alpha-maltopentaosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 maltoheptaose preferred substrate, ratio of transglycosylation to hydrolysis is 100:0.2, recombinant enzyme Saccharolobus solfataricus P2 alpha-maltopentaosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 maltohexaose ratio of transglycosylation to hydrolysis is 90:0.2, recombinant enzyme Saccharolobus solfataricus alpha-maltotetraosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 maltohexaose ratio of transglycosylation to hydrolysis is 90:0.2, recombinant enzyme Saccharolobus solfataricus P2 alpha-maltotetraosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 Maltopentaose ratio of transglycosylation to hydrolysis is 77:0.6, recombinant enzyme Saccharolobus solfataricus alpha-Maltotriosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 Maltopentaose ratio of transglycosylation to hydrolysis is 77:0.6, recombinant enzyme Saccharolobus solfataricus P2 alpha-Maltotriosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 maltotetraose ratio of transglycosylation to hydrolysis is 38:0.6, recombinant enzyme Saccharolobus solfataricus alpha-maltosyl trehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 maltotriose ratio of transglycosylation to hydrolysis is 10:1.4, recombinant enzyme Saccharolobus solfataricus alpha-glucosyltrehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 maltotriose ratio of transglycosylation to hydrolysis is 10:1.4, recombinant enzyme Saccharolobus solfataricus P2 alpha-glucosyltrehalose i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp ?
5.4.99.15 additional information mainly catalyzes an intramolecular transglycosyl reaction to form trehalosyl dextrins from dextrins by converting the alpha-1,4-glucosidic linkage at the reducing end to an alpha-1,1-glucosidic linkage Saccharolobus solfataricus ?
-
?
5.4.99.15 additional information mainly catalyzes an intramolecular transglycosyl reaction to form trehalosyl dextrins from dextrins by converting the alpha-1,4-glucosidic linkage at the reducing end to an alpha-1,1-glucosidic linkage Saccharolobus solfataricus P2 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.4.99.15 ? x * 87000, SDS-PAGE Saccharolobus solfataricus
5.4.99.15 ? x * 86609, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
5.4.99.15 SSO2095 locus name Saccharolobus solfataricus
5.4.99.15 TDFE
-
Saccharolobus solfataricus
5.4.99.15 trehalosyl dextrin-forming enzyme
-
Saccharolobus solfataricus
5.4.99.15 TreY
-
Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.15 75
-
-
Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.99.15 45 90 45°C: about 60% of maximal activity, 90°C: about 55% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.15 80
-
2 h, activity remains unchanged Saccharolobus solfataricus
5.4.99.15 85
-
2 h, 45% loss of activity Saccharolobus solfataricus
5.4.99.15 95
-
2 h, 90% loss of activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.15 5
-
-
Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.15 4.3 6 pH 4.3: about 75% of maximal activity, pH 6.0: about 80% of maximal activity Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.15 4.5 11 24 h, stable Saccharolobus solfataricus