EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.15 | cloned from the genomic DNA of Sulfolobus solfataricus ATCC 35092 to an expression vector with a T7 lac promoter and then expressed in Escherichia coli | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.15 | Cu2+ | 4 mM, about 40% loss of activity | Saccharolobus solfataricus | |
5.4.99.15 | additional information | Ca2+, K+, Mg2+, or Na+ have no activating or inhibiting effect on activity | Saccharolobus solfataricus | |
5.4.99.15 | Zn2+ | 4 mM, about 60% loss of activity | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.15 | additional information | metal ions are not required for activity. Ca2+, K+, Mg2+, or Na+ have no activating or inhibiting effect on activity | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 86609 | - |
x * 86609, calculated from sequence | Saccharolobus solfataricus |
5.4.99.15 | 87000 | - |
x * 87000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.15 | Saccharolobus solfataricus | Q7LX98 | - |
- |
5.4.99.15 | Saccharolobus solfataricus P2 | Q7LX98 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.15 | - |
Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 19 | - |
pH 5.0, 75°C, substrate: maltohexaose | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.15 | maltoheptaose | preferred substrate, ratio of transglycosylation to hydrolysis is 100:0.2, recombinant enzyme | Saccharolobus solfataricus | alpha-maltopentaosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | maltoheptaose | preferred substrate, ratio of transglycosylation to hydrolysis is 100:0.2, recombinant enzyme | Saccharolobus solfataricus P2 | alpha-maltopentaosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | maltohexaose | ratio of transglycosylation to hydrolysis is 90:0.2, recombinant enzyme | Saccharolobus solfataricus | alpha-maltotetraosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | maltohexaose | ratio of transglycosylation to hydrolysis is 90:0.2, recombinant enzyme | Saccharolobus solfataricus P2 | alpha-maltotetraosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | Maltopentaose | ratio of transglycosylation to hydrolysis is 77:0.6, recombinant enzyme | Saccharolobus solfataricus | alpha-Maltotriosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | Maltopentaose | ratio of transglycosylation to hydrolysis is 77:0.6, recombinant enzyme | Saccharolobus solfataricus P2 | alpha-Maltotriosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | maltotetraose | ratio of transglycosylation to hydrolysis is 38:0.6, recombinant enzyme | Saccharolobus solfataricus | alpha-maltosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | maltotriose | ratio of transglycosylation to hydrolysis is 10:1.4, recombinant enzyme | Saccharolobus solfataricus | alpha-glucosyltrehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | maltotriose | ratio of transglycosylation to hydrolysis is 10:1.4, recombinant enzyme | Saccharolobus solfataricus P2 | alpha-glucosyltrehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
5.4.99.15 | additional information | mainly catalyzes an intramolecular transglycosyl reaction to form trehalosyl dextrins from dextrins by converting the alpha-1,4-glucosidic linkage at the reducing end to an alpha-1,1-glucosidic linkage | Saccharolobus solfataricus | ? | - |
? | |
5.4.99.15 | additional information | mainly catalyzes an intramolecular transglycosyl reaction to form trehalosyl dextrins from dextrins by converting the alpha-1,4-glucosidic linkage at the reducing end to an alpha-1,1-glucosidic linkage | Saccharolobus solfataricus P2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.99.15 | ? | x * 87000, SDS-PAGE | Saccharolobus solfataricus |
5.4.99.15 | ? | x * 86609, calculated from sequence | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.15 | SSO2095 | locus name | Saccharolobus solfataricus |
5.4.99.15 | TDFE | - |
Saccharolobus solfataricus |
5.4.99.15 | trehalosyl dextrin-forming enzyme | - |
Saccharolobus solfataricus |
5.4.99.15 | TreY | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 75 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 45 | 90 | 45°C: about 60% of maximal activity, 90°C: about 55% of maximal activity | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 80 | - |
2 h, activity remains unchanged | Saccharolobus solfataricus |
5.4.99.15 | 85 | - |
2 h, 45% loss of activity | Saccharolobus solfataricus |
5.4.99.15 | 95 | - |
2 h, 90% loss of activity | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 5 | - |
- |
Saccharolobus solfataricus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 4.3 | 6 | pH 4.3: about 75% of maximal activity, pH 6.0: about 80% of maximal activity | Saccharolobus solfataricus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.15 | 4.5 | 11 | 24 h, stable | Saccharolobus solfataricus |