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Literature summary extracted from

  • Limauro, D.; De Simone, G.; Pirone, L.; Bartolucci, S.; D'Ambrosio, K.; Pedone, E.
    Sulfolobus solfataricus thiol redox puzzle: characterization of an atypical protein disulfide oxidoreductase (2013), Extremophiles, 18, 219-228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.99.B1 expressed in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.99.B1 the X-ray crystallographic structure is solved at 1.80 A resolution Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.B1 19930
-
 1 * 19930, calculated from sequence Saccharolobus solfataricus
1.8.99.B1 20995
-
 1 * 20995, light scattering and electrospray mass spectroscopy analyses Saccharolobus solfataricus
1.8.99.B1 21000
-
 light scattering and electrospray mass spectroscopy analyses Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.99.B1 protein-dithiol + oxidized acceptor Saccharolobus solfataricus the enzyme is involved in disulfide bond formation protein-disulfide + reduced acceptor
-
 ?
1.8.99.B1 protein-dithiol + oxidized acceptor Saccharolobus solfataricus P2 the enzyme is involved in disulfide bond formation protein-disulfide + reduced acceptor
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.8.99.B1 guanidine-HCl [GuHCl]/2 value is 1.5 M Saccharolobus solfataricus
1.8.99.B1 urea resistant to urea presenting a [urea]/2 value of 5 M Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.B1 Saccharolobus solfataricus Q97Z21
-
-
1.8.99.B1 Saccharolobus solfataricus P2 Q97Z21
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.B1
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.B1 protein-dithiol + oxidized acceptor the enzyme is involved in disulfide bond formation Saccharolobus solfataricus protein-disulfide + reduced acceptor
-
 ?
1.8.99.B1 protein-dithiol + oxidized acceptor the enzyme is involved in disulfide bond formation Saccharolobus solfataricus P2 protein-disulfide + reduced acceptor
-
 ?
1.8.99.B1 [insulin]-disulfide + reduced dithiothreitol the enzyme does not present isomerase activity Saccharolobus solfataricus [insulin]-dithiol + oxidized dithiothreitol
-
 ?
1.8.99.B1 [insulin]-disulfide + reduced dithiothreitol the enzyme does not present isomerase activity Saccharolobus solfataricus P2 [insulin]-dithiol + oxidized dithiothreitol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.99.B1 monomer 1 * 19930, calculated from sequence Saccharolobus solfataricus
1.8.99.B1 monomer 1 * 20995, light scattering and electrospray mass spectroscopy analyses Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.8.99.B1 Sso1120
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.99.B1 90
-
 Tm-value Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.99.B1 Saccharolobus solfataricus calculated from sequence
-
 5.3