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Literature summary extracted from

  • Prangishvilli, D.; Zillig, W.; Gierl, A.; Biesert, L.; Holz, I.
    DNA-dependent RNA polymerase of thermoacidophilic archaebacteria (1982), Eur. J. Biochem., 122, 471-477.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 additional information no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin Desulfurococcus mucosus
2.7.7.6 additional information no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin Sulfolobus acidocaldarius
2.7.7.6 additional information no inhibition by 0.1 mg/ml of rifampicin, streptolydigin or alpha-amanitin Thermoproteus tenax
2.7.7.6 oxygen the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite Thermoproteus tenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 K+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Sulfolobus acidocaldarius
2.7.7.6 K+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Thermoproteus tenax
2.7.7.6 K+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Desulfurococcus mucosus
2.7.7.6 Mg2+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Sulfolobus acidocaldarius
2.7.7.6 Mg2+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Thermoproteus tenax
2.7.7.6 Mg2+ optimal activity at 0.02 M MgCl2 and 0.1 M KCl Desulfurococcus mucosus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Desulfurococcus mucosus
-
-
-
2.7.7.6 Desulfurococcus mucosus 07
-
-
-
2.7.7.6 Sulfolobus acidocaldarius
-
-
-
2.7.7.6 Sulfolobus acidocaldarius DSM 639
-
-
-
2.7.7.6 Thermoproteus tenax
-
-
-
2.7.7.6 Thermoproteus tenax DSM 2078
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.7.6 the enzyme is highly oxygen sensitive. Inactivation is accompanied by cross-linking of components. Inactivated enzyme can be reactivated by reduction with sodium dithionite Thermoproteus tenax

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Sulfolobus acidocaldarius
2.7.7.6
-
 Thermoproteus tenax
2.7.7.6
-
 Desulfurococcus mucosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn DNA-template dependent reaction. T7 DNA and the plasmid PBR322 are by far the best templates. P2, T4 and T5 DNA are weak templates Desulfurococcus mucosus diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn DNA-template dependent reaction. The best of the templates is phiH DNA, whereas T7 and T4 DNA are comparatively inactive and P2 DNA is a very weak template Sulfolobus acidocaldarius diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn DNA-template dependent reaction. The enzyme transcribes phiH DNA as efficiently and T4 DNA as weakly as the Sulfolobus enzyme but T7 DNA even better than phiH DNA Thermoproteus tenax diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn DNA-template dependent reaction. The best of the templates is phiH DNA, whereas T7 and T4 DNA are comparatively inactive and P2 DNA is a very weak template Sulfolobus acidocaldarius DSM 639 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn DNA-template dependent reaction. The enzyme transcribes phiH DNA as efficiently and T4 DNA as weakly as the Sulfolobus enzyme but T7 DNA even better than phiH DNA Thermoproteus tenax DSM 2078 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn DNA-template dependent reaction. T7 DNA and the plasmid PBR322 are by far the best templates. P2, T4 and T5 DNA are weak templates Desulfurococcus mucosus 07 diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 More components of the RNA polymerase and their molecular weights determined by SDS-PAGE Sulfolobus acidocaldarius
2.7.7.6 More components of the RNA polymerase and their molecular weights determined by SDS-PAGE Thermoproteus tenax
2.7.7.6 More components of the RNA polymerase and their molecular weights determined by SDS-PAGE Desulfurococcus mucosus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 DNA-dependent RNA polymerase
-
 Sulfolobus acidocaldarius
2.7.7.6 DNA-dependent RNA polymerase
-
 Thermoproteus tenax
2.7.7.6 DNA-dependent RNA polymerase
-
 Desulfurococcus mucosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 85
-
 assay at Sulfolobus acidocaldarius
2.7.7.6 85
-
 assay at Thermoproteus tenax
2.7.7.6 85
-
 assay at Desulfurococcus mucosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 8
-
 assay at Sulfolobus acidocaldarius
2.7.7.6 8
-
 assay at Thermoproteus tenax
2.7.7.6 8
-
 assay at Desulfurococcus mucosus
2.7.7.6 8 8.5
-
 Sulfolobus acidocaldarius
2.7.7.6 8 8.5
-
 Thermoproteus tenax
2.7.7.6 8 8.5
-
 Desulfurococcus mucosus