EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B28 | 46 | - |
pentyl beta-D-glucopyranoside | pH 5.0, 80°C, 6% water, 94% hexanol | Pyrococcus furiosus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.2.1.B28 | hexanol | enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Caldicellulosiruptor saccharolyticus | - |
- |
- |
3.2.1.21 | Prunus dulcis | - |
- |
- |
3.2.1.21 | Pyrococcus furiosus | - |
- |
- |
3.2.1.23 | Escherichia coli | - |
- |
- |
3.2.1.23 | Saccharolobus solfataricus | - |
- |
- |
3.2.1.B28 | Pyrococcus furiosus | Q51723 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | the enzyme catalysed both hydrolysis and transglycosylation | Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | the enzyme catalysed both hydrolysis and transglycosylation | Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | the enzyme catalyses both hydrolysis and transglycosylation | Prunus dulcis | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | additional information | the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 | Pyrococcus furiosus | ? | - |
? | |
3.2.1.21 | additional information | the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 | Prunus dulcis | ? | - |
? | |
3.2.1.21 | additional information | the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.23 | 4-nitrophenyl beta-D-galactoside + H2O | the enzyme catalysed both hydrolysis and transglycosylation | Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | 4-nitrophenyl beta-D-galactoside + H2O | the enzyme catalyses both hydrolysis and transglycosylation | Escherichia coli | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | additional information | the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 | Escherichia coli | ? | - |
? | |
3.2.1.23 | additional information | the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 | Saccharolobus solfataricus | ? | - |
? | |
3.2.1.B28 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + D-galactose | - |
? | |
3.2.1.B28 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
3.2.1.B28 | additional information | enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity | Pyrococcus furiosus | ? | - |
? | |
3.2.1.B28 | pentyl beta-D-glucopyranoside + H2O | - |
Pyrococcus furiosus | pentanol + beta-D-glucopyranose | - |
? |