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Literature summary extracted from

  • Hansson, T.; Andersson, M.; Wehtje, E.; Adlercreutz, P.
    Influence of water activity on the competition between beta-glycosidase-catalysed transglycosylation and hydrolysis in aqueous hexanol (2001), Enzyme Microb. Technol., 29, 527-534.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B28 46
-
pentyl beta-D-glucopyranoside pH 5.0, 80°C, 6% water, 94% hexanol Pyrococcus furiosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.B28 hexanol enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Caldicellulosiruptor saccharolyticus
-
-
-
3.2.1.21 Prunus dulcis
-
-
-
3.2.1.21 Pyrococcus furiosus
-
-
-
3.2.1.23 Escherichia coli
-
-
-
3.2.1.23 Saccharolobus solfataricus
-
-
-
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O the enzyme catalysed both hydrolysis and transglycosylation Caldicellulosiruptor saccharolyticus 4-nitrophenol + beta-D-glucose
-
?
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O the enzyme catalysed both hydrolysis and transglycosylation Pyrococcus furiosus 4-nitrophenol + beta-D-glucose
-
?
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O the enzyme catalyses both hydrolysis and transglycosylation Prunus dulcis 4-nitrophenol + beta-D-glucose
-
?
3.2.1.21 additional information the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 Pyrococcus furiosus ?
-
?
3.2.1.21 additional information the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 Prunus dulcis ?
-
?
3.2.1.21 additional information the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 Caldicellulosiruptor saccharolyticus ?
-
?
3.2.1.23 4-nitrophenyl beta-D-galactoside + H2O the enzyme catalysed both hydrolysis and transglycosylation Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
?
3.2.1.23 4-nitrophenyl beta-D-galactoside + H2O the enzyme catalyses both hydrolysis and transglycosylation Escherichia coli 4-nitrophenol + beta-D-galactose
-
?
3.2.1.23 additional information the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 Escherichia coli ?
-
?
3.2.1.23 additional information the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0 Saccharolobus solfataricus ?
-
?
3.2.1.B28 4-nitrophenyl beta-D-galactopyranoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + D-galactose
-
?
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + beta-D-glucopyranose
-
?
3.2.1.B28 additional information enzyme catalyzes both hydrolysis and transglycosylation of glycosidc substrates. In hexanol/water two-phase systems, hydrolysis is by far the dominating reaction even though the total activity increases. In hexanol containing various amounts of water, the selectivity for the alcohol increases with increasing water activity. This counteracts the effect of higher water concentration and the transglycosylation/hydrolysis ratio increases with increasing water activity Pyrococcus furiosus ?
-
?
3.2.1.B28 pentyl beta-D-glucopyranoside + H2O
-
Pyrococcus furiosus pentanol + beta-D-glucopyranose
-
?