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Literature summary extracted from

  • Seybert, A.; Singleton, M.R.; Cook, N.; Hall, D.R.; Wigley, D.B.
    Communication between subunits within an archaeal clamp-loader complex (2006), EMBO J., 25, 2209-2218.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.4.B8 crystal structure of replication factor C domains 1 and 2 at 2.1 A, crystal structure of replication factor C ADPNP complex at 3.5 A and at 4.0 A, hanging drop vapour diffusion method Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.B8 additional information mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.4.B8 additional information
-
 ATP Km-values of mutant replication factor C complexes in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA) Archaeoglobus fulgidus
3.6.4.B8 0.025
-
 ATP wild-type enzyme, in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA), pH and temperature not specified in the publication Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.B8 Archaeoglobus fulgidus O28219 and O29072 O28219: Replication factor C small subunit, O29072: Replication factor C large subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.B8
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B8 ATP + H2O
-
 Archaeoglobus fulgidus ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.4.B8 oligomer communication between subunits in the RFC holoenzyme is investigated. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. This symmetry is broken when the nucleotide is bound to the complex. The large conformational change observed may relate to the opening of PCNA rings that is required for them to be loaded onto DNA substrates Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
3.6.4.B8 replication factor C
-
 Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.4.B8 additional information
-
 ATP turnover numbers of mutant replication factor C complexes in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA) Archaeoglobus fulgidus
3.6.4.B8 1.7
-
 ATP wild-type enzyme, in the presence of DNA and Proliferating-Cell-Nuclear-Antigen (PCNA), pH and temperature not specified in the publication Archaeoglobus fulgidus