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Literature summary extracted from

  • Bchini, R.; Vasiliou, V.; Branlant, G.; Talfournier, F.; Rahuel-Clermont, S.
    Retinoic acid biosynthesis catalyzed by retinal dehydrogenases relies on a rate-limiting conformational transition associated with substrate recognition (2013), Chem. Biol. Interact., 202, 78-84.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36 expressed in Escherichia coli C41(DE3) cells Homo sapiens
1.2.1.36 expressed in Escherichia coli C41(DE3) cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.36 K127A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
1.2.1.36 N120A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
1.2.1.36 Y296A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
1.2.1.36 Y296V the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.002
-
 retinal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 0.0029
-
 citral wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 0.0063
-
 2,4-decadienal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 0.0064
-
 decanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 0.0081
-
 retinal wild type enzyme ALDH1A1, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.0086
-
 retinal mutant enzyme K127A, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.0089
-
 retinal mutant enzyme Y296A, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.01
-
 decanal wild type enzyme ALDH1A1, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.011
-
 retinal mutant enzyme N120A, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.015
-
 retinal mutant enzyme Y296V, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.023
-
 hexanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.36 retinal + NAD+ + H2O Homo sapiens
-
 retinoate + NADH + H+
-
 ?
1.2.1.36 retinal + NAD+ + H2O Rattus norvegicus
-
 retinoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Homo sapiens
-
-
-
1.2.1.36 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36 nickel-Sepharose column chromatography and Q-Sepharose column chromatography Homo sapiens
1.2.1.36 nickel-Sepharose column chromatography and Q-Sepharose column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 2,4-decadienal + NAD+ + H2O
-
 Homo sapiens 2,4-decadienoate + NADH + H+
-
 ?
1.2.1.36 2,4-decadienal + NAD+ + H2O
-
 Rattus norvegicus 2,4-decadienoate + NADH + H+
-
 ?
1.2.1.36 citral + NAD+ + H2O
-
 Homo sapiens (2E)-3,7-dimethylocta-2,6-dienoic acid + NADH + H+
-
 ?
1.2.1.36 citral + NAD+ + H2O
-
 Rattus norvegicus (2E)-3,7-dimethylocta-2,6-dienoic acid + NADH + H+
-
 ?
1.2.1.36 decanal + NAD+ + H2O
-
 Homo sapiens decanoate + NADH + H+
-
 ?
1.2.1.36 decanal + NAD+ + H2O
-
 Rattus norvegicus decanoate + NADH + H+
-
 ?
1.2.1.36 hexanal + NAD+ + H2O
-
 Homo sapiens hexanoate + NADH + H+
-
 ?
1.2.1.36 hexanal + NAD+ + H2O
-
 Rattus norvegicus hexanoate + NADH + H+
-
 ?
1.2.1.36 retinal + NAD+ + H2O
-
 Homo sapiens retinoate + NADH + H+
-
 ?
1.2.1.36 retinal + NAD+ + H2O
-
 Rattus norvegicus retinoate + NADH + H+
-
 ?
1.2.1.36 retinol + NAD+ + H2O
-
 Homo sapiens ? + NADH + H+
-
 ?
1.2.1.36 retinol + NAD+ + H2O
-
 Rattus norvegicus ? + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.36 ALDH1A1
-
 Homo sapiens
1.2.1.36 Aldh1a2
-
 Rattus norvegicus
1.2.1.36 NAD-dependent retinal dehydrogenase
-
 Rattus norvegicus
1.2.1.36 NAD-dependent retinal dehydrogenases
-
 Homo sapiens
1.2.1.36 RalDH1
-
 Homo sapiens
1.2.1.36 RALDH2
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.36 0.008
-
 retinal mutant enzyme Y296V, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.017
-
 retinal mutant enzyme Y296A, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.03
-
 retinal mutant enzyme K127A, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.03
-
 retinal mutant enzyme N120A, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.07
-
 retinal wild type enzyme ALDH1A1, at pH 8.5 and 25°C Homo sapiens
1.2.1.36 0.1
-
 retinal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 0.1
-
 citral wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 0.15
-
 2,4-decadienal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 2
-
 hexanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus
1.2.1.36 2.2
-
 decanal wild type enzyme ALDH1A2, at pH 8.5 and 25°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 NAD+
-
 Homo sapiens
1.2.1.36 NAD+
-
 Rattus norvegicus