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Literature summary extracted from

  • Gonzalez-Segura, L.; Ho, K.K.; Perez-Miller, S.; Weiner, H.; Hurley, T.D.
    Catalytic contribution of threonine 244 in human ALDH2 (2013), Chem. Biol. Interact., 202, 32-40.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.5 expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.5 the apoenzyme is crystallized by using 100 mM ACES, pH 6.4, 100-200 mM guanidine-HCl, 1-10 mM MgCl2 and 16–17% (w/v) PEG 6000, the mutant T244A in complex with NAD is crystallized by using 100 mM ACES, pH 6.4, 100 mM guanidine–HCl, 10 mM MgCl2 and 18% (w/v) PEG 6000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.5 T244A the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens
1.2.1.5 T244G the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens
1.2.1.5 T244S the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens
1.2.1.5 T244V the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.5 0.013
-
 Chloroacetaldehyde mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.039
-
 Chloroacetaldehyde wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.048
-
 NAD+ wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.052
-
 Chloroacetaldehyde mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.094
-
 NAD+ mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.19
-
 NAD+ mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.3
-
 Chloroacetaldehyde mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.39
-
 NAD+ mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.46
-
 Chloroacetaldehyde mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 2.1
-
 NAD+ mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.5 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.5 Mg2+ stimulatory effect at 5 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 55000
-
 4 * 55000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.5 chloroacetaldehyde + NAD+ + H2O Homo sapiens
-
 chloroacetate + NADH + H+
-
 ?
1.2.1.5 propionaldehyde + NAD+ + H2O Homo sapiens
-
 propionate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.5 DEAE-cellulose column chromatography, 5’-AMP Sepharose 4B and 4-hydroxyacetophenone affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.5 chloroacetaldehyde + NAD+ + H2O
-
 Homo sapiens chloroacetate + NADH + H+
-
 ?
1.2.1.5 propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.5 homotetramer 4 * 55000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.2.1.5 ALDH2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.5 0.47
-
 NAD+ mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.47
-
 Chloroacetaldehyde mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.63
-
 NAD+ mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 0.63
-
 Chloroacetaldehyde mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 2.5
-
 NAD+ mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 2.5
-
 Chloroacetaldehyde mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 4
-
 NAD+ mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 4
-
 Chloroacetaldehyde mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 7.7
-
 NAD+ wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 7.7
-
 Chloroacetaldehyde wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.5 NAD(P)+
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.5 1.8
-
 NAD+ mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 3.3
-
 NAD+ mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 5.5
-
 Chloroacetaldehyde mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 6.5
-
 NAD+ mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 12.2
-
 Chloroacetaldehyde mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 13.2
-
 Chloroacetaldehyde mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 33.3
-
 NAD+ mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 158.3
-
 NAD+ wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 183.3
-
 Chloroacetaldehyde mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens
1.2.1.5 200
-
 Chloroacetaldehyde wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication Homo sapiens