EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.5 | expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.5 | the apoenzyme is crystallized by using 100 mM ACES, pH 6.4, 100-200 mM guanidine-HCl, 1-10 mM MgCl2 and 1617% (w/v) PEG 6000, the mutant T244A in complex with NAD is crystallized by using 100 mM ACES, pH 6.4, 100 mM guanidineHCl, 10 mM MgCl2 and 18% (w/v) PEG 6000 | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.5 | T244A | the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme | Homo sapiens |
1.2.1.5 | T244G | the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme | Homo sapiens |
1.2.1.5 | T244S | the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme | Homo sapiens |
1.2.1.5 | T244V | the mutant exhibits reduced catalytic efficiency toward substrate and coenzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.5 | 0.013 | - |
Chloroacetaldehyde | mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.039 | - |
Chloroacetaldehyde | wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.048 | - |
NAD+ | wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.052 | - |
Chloroacetaldehyde | mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.094 | - |
NAD+ | mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.19 | - |
NAD+ | mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.3 | - |
Chloroacetaldehyde | mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.39 | - |
NAD+ | mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.46 | - |
Chloroacetaldehyde | mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 2.1 | - |
NAD+ | mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.5 | mitochondrion | - |
Homo sapiens | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.5 | Mg2+ | stimulatory effect at 5 mM | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.5 | 55000 | - |
4 * 55000, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.5 | chloroacetaldehyde + NAD+ + H2O | Homo sapiens | - |
chloroacetate + NADH + H+ | - |
? | |
1.2.1.5 | propionaldehyde + NAD+ + H2O | Homo sapiens | - |
propionate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.5 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.5 | DEAE-cellulose column chromatography, 5-AMP Sepharose 4B and 4-hydroxyacetophenone affinity chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.5 | chloroacetaldehyde + NAD+ + H2O | - |
Homo sapiens | chloroacetate + NADH + H+ | - |
? | |
1.2.1.5 | propionaldehyde + NAD+ + H2O | - |
Homo sapiens | propionate + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.5 | homotetramer | 4 * 55000, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.5 | ALDH2 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.5 | 0.47 | - |
NAD+ | mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.47 | - |
Chloroacetaldehyde | mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.63 | - |
NAD+ | mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 0.63 | - |
Chloroacetaldehyde | mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 2.5 | - |
NAD+ | mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 2.5 | - |
Chloroacetaldehyde | mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 4 | - |
NAD+ | mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 4 | - |
Chloroacetaldehyde | mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 7.7 | - |
NAD+ | wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 7.7 | - |
Chloroacetaldehyde | wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.5 | NAD(P)+ | - |
Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.5 | 1.8 | - |
NAD+ | mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 3.3 | - |
NAD+ | mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 5.5 | - |
Chloroacetaldehyde | mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 6.5 | - |
NAD+ | mutant enzyme T244V, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 12.2 | - |
Chloroacetaldehyde | mutant enzyme T244G, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 13.2 | - |
Chloroacetaldehyde | mutant enzyme T244A, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 33.3 | - |
NAD+ | mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 158.3 | - |
NAD+ | wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 183.3 | - |
Chloroacetaldehyde | mutant enzyme T244S, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens | |
1.2.1.5 | 200 | - |
Chloroacetaldehyde | wild type enzyme, in 25 mM Na-HEPES buffer (pH 7.4), temperature not specified in the publication | Homo sapiens |