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Literature summary extracted from

  • Brzezinska, M.; Szulc, I.; Brzostek, A.; Klink, M.; Kielbik, M.; Sulowska, Z.; Pawelczyk, J.; Dziadek, J.
    The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis (2013), BMC Microbiol., 13, 43.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Mycobacterium tuberculosis
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 gene kstD
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1.3.99.4 Mycobacterium tuberculosis H37Rv
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 gene kstD
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Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 3-ketosteroid 1(2)-dehydrogenase
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 Mycobacterium tuberculosis
1.3.99.4 KstD
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 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
1.3.99.4 Mycobacterium tuberculosis construction of a mutant Mtb H37Rv strain containing an inactivated kstD gene, DELTAkstD, which encodes 3-ketosteroid 1(2)-dehydrogenase by homologous recombination-based gene-replacement technique. Replication of mutant Mycobacterium tuberculosis is attenuated in resting human macrophages compared to the wild-type or complemented strains. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages. But mutant and wild-type Mycobacterium tuberculosis behave similarly in macrophages activated with IFN-gamma before and during infection. The mutant is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply additional information

General Information

EC Number General Information Comment Organism
1.3.99.4 malfunction an enzyme-deficient mutant strain is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages, phenotpe, overview Mycobacterium tuberculosis