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Literature summary extracted from
- Preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase from Pyrococcus furiosus (2013), BMC Biotechnol., 13, 73.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | synthesis | preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase | Pyrococcus furiosus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | cloned in frame with the Saccharomyces cerecisiae a-factor secretory signal and expressed in Pichia pastoris strain X-33 | Pyrococcus furiosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Cu2+ | 1 mM, 22% inhibition, not significantly stimulated or inhibited by other divalent cations | Pyrococcus furiosus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 120000 | - |
dimer | Pyrococcus furiosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Pyrococcus furiosus | Q51723 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | - |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 694.6 | - |
pH and temperature not specified in the publication | Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | lactose + H2O | preparation of lactose-free pasteurized milk with a recombinant thermostable beta-glucosidase | Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 100 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 70 | 110 | 70°C: about 45% of maximal activity, 110°C: about 30% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 30 | 120 | the enzyme retains more than 80% of its maximum activity from 30°C to 120°C | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6 | - |
- |
Pyrococcus furiosus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | 7 | pH 5.0: about 60% of maximal activity, pH 7.0: about 80% of maximal activity | Pyrococcus furiosus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | 8 | 37°C, 1 h incubation, the enzyme retains more than 80% of its maximum activity | Pyrococcus furiosus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Pyrococcus furiosus | - |
- |
4.4 |
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