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Literature summary extracted from

  • Faccio, G.; Kruus, K.; Buchert, J.; Saloheimo, M.
    Secreted fungal sulfhydryl oxidases: Sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae (2010), BMC Biochem., 11, 31.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.2 expression in Trichoderma reesei Aspergillus oryzae
1.8.3.3 expression in Trichoderma reesei Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.2 KI 1 mM, 65% residual activity Aspergillus oryzae
1.8.3.2 MgSO4 1 mM, 55% residual activity Aspergillus oryzae
1.8.3.2 Na2SO4 1 mM, 61% residual activity Aspergillus oryzae
1.8.3.2 sodium dodecylsulfate 1 mM, 58% residual activity Aspergillus oryzae
1.8.3.2 Urea 1 mM, 80% residual activity Aspergillus oryzae
1.8.3.2 ZnSO4 1 mM, complete inhibition Aspergillus oryzae
1.8.3.3 KI 1 mM, 65% residual activity Aspergillus oryzae
1.8.3.3 MgSO4 1 mM, 55% residual activity Aspergillus oryzae
1.8.3.3 Na2SO4 1 mM, 61% residual activity Aspergillus oryzae
1.8.3.3 sodium dodecylsulfate 1 mM, 58% residual activity Aspergillus oryzae
1.8.3.3 Urea 1 mM, 80% residual activity Aspergillus oryzae
1.8.3.3 ZnSO4 1 mM, complete inhibition Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.2 1.55
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 2.41
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 2.78
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 6.11
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 9.73
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 1.55
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 2.41
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 2.78
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 6.11
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 9.73
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 43959
-
 x * 45000, SDS-PAGE, x * 43959, mass spectrometry Aspergillus oryzae
1.8.3.2 45000
-
 x * 45000, SDS-PAGE, x * 43959, mass spectrometry Aspergillus oryzae
1.8.3.3 43959
-
 x * 45000, SDS-PAGE, x * 43959, mass spectrometry Aspergillus oryzae
1.8.3.3 45000
-
 x * 45000, SDS-PAGE, x * 43959, mass spectrometry Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Aspergillus oryzae Q2UA33
-
-
1.8.3.2 Aspergillus oryzae ATCC 42149 Q2UA33
-
-
1.8.3.3 Aspergillus oryzae Q2UA33
-
-
1.8.3.3 Aspergillus oryzae ATCC 42149 Q2UA33
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.3.2 glycoprotein sequence contains six potential N-glycosylation site. Deglycosylation reduces the molecluar mass to 40000 Da Aspergillus oryzae
1.8.3.3 glycoprotein sequence contains six potential N-glycosylation site. Deglycosylation reduces the molecluar mass to 40000 Da Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2 recombinant protein Aspergillus oryzae
1.8.3.3 recombinant protein Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 2 2-mercaptoethanol + O2
-
 Aspergillus oryzae (ethyldisulfanyl)ethane + H2O2
-
 ?
1.8.3.2 2 2-mercaptoethanol + O2
-
 Aspergillus oryzae ATCC 42149 (ethyldisulfanyl)ethane + H2O2
-
 ?
1.8.3.2 2 D-Cys + O2
-
 Aspergillus oryzae D-cystine + H2O2
-
 ?
1.8.3.2 2 D-Cys + O2
-
 Aspergillus oryzae ATCC 42149 D-cystine + H2O2
-
 ?
1.8.3.2 2 dithiothreitol + O2
-
 Aspergillus oryzae dithiothreitol disulfide + H2O2
-
 ?
1.8.3.2 2 dithiothreitol + O2
-
 Aspergillus oryzae ATCC 42149 dithiothreitol disulfide + H2O2
-
 ?
1.8.3.2 2 glutathione + O2
-
 Aspergillus oryzae glutathione disulfide + H2O2
-
 ?
1.8.3.2 2 glutathione + O2
-
 Aspergillus oryzae ATCC 42149 glutathione disulfide + H2O2
-
 ?
1.8.3.2 2 L-Cys + O2
-
 Aspergillus oryzae L-cystine + H2O2
-
 ?
1.8.3.2 2 L-Cys + O2
-
 Aspergillus oryzae ATCC 42149 L-cystine + H2O2
-
 ?
1.8.3.3 2 2-mercaptoethanol + O2
-
 Aspergillus oryzae (ethyldisulfanyl)ethane + H2O2
-
 ?
1.8.3.3 2 D-Cys + O2
-
 Aspergillus oryzae D-cystine + H2O2
-
 ?
1.8.3.3 2 dithiothreitol + O2
-
 Aspergillus oryzae dithiothreitol disulfide + H2O2
-
 ?
1.8.3.3 2 glutathione + O2
-
 Aspergillus oryzae glutathione disulfide + H2O2
-
 ?
1.8.3.3 2 L-Cys + O2
-
 Aspergillus oryzae L-cystine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.2 ? x * 45000, SDS-PAGE, x * 43959, mass spectrometry Aspergillus oryzae
1.8.3.3 ? x * 45000, SDS-PAGE, x * 43959, mass spectrometry Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.8.3.2 Sox1
-
 Aspergillus oryzae
1.8.3.3 Sox1
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.2 40
-
 24 h, more than 70% of initial activity Aspergillus oryzae
1.8.3.2 50
-
 1 h, 40% of initial activity Aspergillus oryzae
1.8.3.2 57
-
 melting tepmerature Aspergillus oryzae
1.8.3.3 40
-
 24 h, more than 70% of initial activity Aspergillus oryzae
1.8.3.3 50
-
 1 h, 40% of initial activity Aspergillus oryzae
1.8.3.3 57
-
 melting temperature Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.2 50 100 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 5950
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 52300
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 309000
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 3600000
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 50 100 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 5950
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 52300
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 309000
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 3600000
-
 glutathione pH 7.5, 20°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 8
-
-
 Aspergillus oryzae
1.8.3.3 8
-
-
 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.3.2 4 8 24 h, more than 80% of initial activity Aspergillus oryzae
1.8.3.3 4 8 24 h, more than 80% of initial activity Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.3.2 3840
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 8560
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 128000
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 515000
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 1290000
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 3840
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 8560
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 128000
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 515000
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.3 1290000
-
 glutathione pH 7.5, 20°C Aspergillus oryzae