EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.9 | expressed in Escherichia coli BL21(DE3) cells | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.00031 | - |
thioredoxin | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 0.00235 | - |
NADPH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 0.0186 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 0.1 | - |
NADH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 36500 | - |
x * 36500, calculated from amino acid sequence | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | thioredoxin + NADP+ | Solanum lycopersicum | - |
thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Solanum lycopersicum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | Ni2+-affinity column chromatography | Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+ | - |
Solanum lycopersicum | 5'-thionitrobenzoic acid + NAD+ | - |
? | |
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Solanum lycopersicum | 5'-thionitrobenzoic acid + NADP+ | - |
? | |
1.8.1.9 | thioredoxin + NADP+ | - |
Solanum lycopersicum | thioredoxin disulfide + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.9 | ? | x * 36500, calculated from amino acid sequence | Solanum lycopersicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | NADPH-dependent thioredoxin reductase | - |
Solanum lycopersicum |
1.8.1.9 | NTR | - |
Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.06 | - |
NADH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 0.35 | - |
NADPH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 0.38 | - |
thioredoxin | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 1.77 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | NADH | - |
Solanum lycopersicum | |
1.8.1.9 | NADPH | the catalytic efficiency with NADPH is 2500fold higher than with NADH | Solanum lycopersicum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.8.1.9 | Solanum lycopersicum | calculated from amino acid sequence | - |
5.3 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.6 | - |
NADH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 95 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 150 | - |
NADPH | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum | |
1.8.1.9 | 1230 | - |
thioredoxin | in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication | Solanum lycopersicum |