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Literature summary extracted from

  • Dai, C.; Wang, M.H.
    Isolation and characterization of thioredoxin and NADPH-dependent thioredoxin reductase from tomato (Solanum lycopersicum) (2011), BMB Rep., 44, 692-697.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli BL21(DE3) cells Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.00031
-
thioredoxin in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 0.00235
-
NADPH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 0.0186
-
5,5'-dithiobis(2-nitrobenzoic acid) in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 0.1
-
NADH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 36500
-
x * 36500, calculated from amino acid sequence Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 thioredoxin + NADP+ Solanum lycopersicum
-
thioredoxin disulfide + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 Ni2+-affinity column chromatography Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
Solanum lycopersicum 5'-thionitrobenzoic acid + NAD+
-
?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
Solanum lycopersicum 5'-thionitrobenzoic acid + NADP+
-
?
1.8.1.9 thioredoxin + NADP+
-
Solanum lycopersicum thioredoxin disulfide + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 ? x * 36500, calculated from amino acid sequence Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 NADPH-dependent thioredoxin reductase
-
Solanum lycopersicum
1.8.1.9 NTR
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 0.06
-
NADH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 0.35
-
NADPH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 0.38
-
thioredoxin in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 1.77
-
5,5'-dithiobis(2-nitrobenzoic acid) in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 NADH
-
Solanum lycopersicum
1.8.1.9 NADPH the catalytic efficiency with NADPH is 2500fold higher than with NADH Solanum lycopersicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.9 Solanum lycopersicum calculated from amino acid sequence
-
5.3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.9 0.6
-
NADH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 95
-
5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 150
-
NADPH in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum
1.8.1.9 1230
-
thioredoxin in 50 mM potassium phosphate, pH 7.0, 1 mM EDTA, temperature not specified in the publication Solanum lycopersicum