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Literature summary extracted from

  • Oh, H.J.; Shin, K.C.; Oh, D.K.
    Production of ginsenosides Rg1 and Rh1 by hydrolyzing the outer glycoside at the C-6 position in protopanaxatriol-type ginsenosides using beta-glucosidase from Pyrococcus furiosus (2013), Biotechnol. Lett., 36, 113-119.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Pyrococcus furiosus Q51723
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 0.05
-
 pH 5.5, 95°C, substrate: ginsenoside Rg2 Pyrococcus furiosus
3.2.1.21 0.06
-
 pH 5.5, 95°C, substrate: ginsenoside R2 Pyrococcus furiosus
3.2.1.21 0.23
-
 pH 5.5, 95°C, substrate: ginsenoside Re Pyrococcus furiosus
3.2.1.21 0.6
-
 pH 5.5, 95°C, substrate: 4-nitrophenyl-alpha-L-rhamnopyranoside Pyrococcus furiosus
3.2.1.21 0.69
-
 pH 5.5, 95°C, substrate: ginsenoside R1 Pyrococcus furiosus
3.2.1.21 6.8
-
 pH 5.5, 95°C, substrate: ginsenoside Rf Pyrococcus furiosus
3.2.1.21 12.5
-
 pH 5.5, 95°C, substrate: 2-nitrophenyl-beta-D-xylopyranoside Pyrococcus furiosus
3.2.1.21 117
-
 pH 5.5, 95°C, substrate: 4-nitrophenyl-beta-D-xylopyranoside Pyrococcus furiosus
3.2.1.21 828
-
 pH 5.5, 95°C, substrate: 2-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus
3.2.1.21 1300
-
 pH 5.5, 95°C, substrate: 4-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.21 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.21 ginsenoside R1 + H2O
-
 Pyrococcus furiosus ginsenoside Rg1 + D-xylose ginsenoside Rg1 i.e. 6alpha,20-bis(beta-D-glucopyranosyl)oxy-dammar-24-en-3beta,12beta-diol ?
3.2.1.21 ginsenoside R2 + H2O
-
 Pyrococcus furiosus ginsenoside Rh1 + D-xylose
-
 ?
3.2.1.21 ginsenoside Re + H2O ginsenoside Re i.e. 20-(beta-D-glucopyranosyl)oxy-6alpha-[alpha-L-rhamnopyranosyl-(1->2)-beta-D-glucopyranosyloxy]dammar-24-en-3beta,12beta-diol Pyrococcus furiosus ginsenoside Rg1 + L-rhamnose ginsenoside Rg1 i.e. 6alpha,20-bis(beta-D-glucopyranosyl)oxy-dammar-24-en-3beta,12beta-diol ?
3.2.1.21 ginsenoside Rf + H2O
-
 Pyrococcus furiosus ginsenoside Rh1 + D-glucose
-
 ?
3.2.1.21 ginsenoside Rg2 + H2O
-
 Pyrococcus furiosus ginsenoside Rh1 + L-rhamnose
-
 ?
3.2.1.21 additional information the enzyme hydrolyzes the outer glycoside at the C-6 position in protopanaxatriol-type ginsenosides whereas the enzyme does not hydrolyze the inner glucoside at the C-6 position and the glucoside at the C-20 position Pyrococcus furiosus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 95
-
 assay at Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
 assay at Pyrococcus furiosus