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Literature summary extracted from

  • Kai, G.; Liu, Y.; Wang, X.; Yang, S.; Fu, X.; Luo, X.; Liao, P.
    Functional identification of hyoscyamine 6beta-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli (2011), Biotechnol. Lett., 33, 1361-1365.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.11 DNA and amino acid sequence determination and analysis, overexpression of N-terminally His- or GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Anisodus acutangulus
1.14.20.13 DNA and amino acid sequence determination and analysis, overexpression of N-terminally His- or GST-tagged enzyme in Escherichia coli strain BL21 (DE3) Anisodus acutangulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.11 43000
-
 x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus
1.14.11.11 64000
-
 x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus
1.14.20.13 43000
-
 x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus
1.14.20.13 64000
-
 x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2 Anisodus acutangulus
-
 (6S)-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.20.13 (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2 Anisodus acutangulus
-
 scopolamine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.11 Anisodus acutangulus
-
-
-
1.14.20.13 Anisodus acutangulus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.11 recombinant N-terminally His- or GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel or glutathione affinity chromatography, respectively Anisodus acutangulus
1.14.20.13 recombinant N-terminally His- or GST-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel or glutathione affinity chromatography, respectively Anisodus acutangulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.11 L-hyoscyamine + 2-oxoglutarate + O2
-
 Anisodus acutangulus (6S)-hydroxyhyoscyamine + succinate + CO2
-
 ?
1.14.11.11 additional information the enzyme also catalyzes the reaction of EC 1.14.11.14 Anisodus acutangulus ?
-
 ?
1.14.20.13 (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
 Anisodus acutangulus scopolamine + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.11 ? x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus
1.14.20.13 ? x * 64000, recombinant GST-tagged enzyme, SDS-PAGE, x * 43000, recombinant His-tagged enzyme, SDS-PAGE Anisodus acutangulus

Synonyms

EC Number Synonyms Comment Organism
1.14.11.11 H6H
-
 Anisodus acutangulus
1.14.11.11 hyoscyamine 6beta-hydroxylase
-
 Anisodus acutangulus
1.14.20.13 H6H
-
 Anisodus acutangulus
1.14.20.13 hyoscyamine 6beta-hydroxylase
-
 Anisodus acutangulus

General Information

EC Number General Information Comment Organism
1.14.11.11 metabolism hyoscyamine 6beta-hydroxylase converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway Anisodus acutangulus
1.14.20.13 metabolism hyoscyamine 6beta-hydroxylase converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway Anisodus acutangulus