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Literature summary extracted from

  • Kamali, N.; Tavallaie, M.; Bambai, B.; Karkhane, A.A.; Miri, M.
    Site-directed mutagenesis enhances the activity of NADH-FMN oxidoreductase (DszD) activity of Rhodococcus erythropolis (2010), Biotechnol. Lett., 32, 921-927.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.42 expressed in Escherichia coli BL21(DE3)pLysS cells Rhodococcus erythropolis

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.42 T62A the mutant shows 7fold increase in activity compared to the wild type enzyme Rhodococcus erythropolis
1.5.1.42 T62N the mutant shows 5fold increase in activity compared to the wild type enzyme Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.42 22000
-
 x * 22000, SDS-PAGE Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.42 FMN + NADH + H+ Rhodococcus erythropolis
-
 FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+ Rhodococcus erythropolis IGTS8 (ATCC 53968)
-
 FMNH2 + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.42 Rhodococcus erythropolis
-
-
-
1.5.1.42 Rhodococcus erythropolis IGTS8 (ATCC 53968)
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.42 FMN + NADH + H+
-
 Rhodococcus erythropolis FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+
-
 Rhodococcus erythropolis IGTS8 (ATCC 53968) FMNH2 + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.42 ? x * 22000, SDS-PAGE Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.42 DszD
-
 Rhodococcus erythropolis
1.5.1.42 NADH-FMN oxidoreductase
-
 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.42 NADH dependent on Rhodococcus erythropolis