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Literature summary extracted from

  • Hansson, T.; Adlercreutz, P.
    The temperature influences the ratio of glucosidase and galactosidase activities of beta-glycosidases (2002), Biotechnol. Lett., 24, 1465-1471.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 4.2
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 90°C Saccharolobus solfataricus
3.2.1.B26 6.2
-
 4-nitrophenyl beta-D-galactoside pH 6.5, 90°C Saccharolobus solfataricus
3.2.1.B26 13
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 70°C Saccharolobus solfataricus
3.2.1.B26 21
-
 4-nitrophenyl beta-D-glucoside pH 6.5, 50°C Saccharolobus solfataricus
3.2.1.B26 21
-
 4-nitrophenyl beta-D-galactoside pH 6.5, 70°C Saccharolobus solfataricus
3.2.1.B26 47
-
 4-nitrophenyl beta-D-galactoside pH 6.5, 50°C Saccharolobus solfataricus
3.2.1.B28 1.3
-
 4-nitrophenyl beta-D-galactoside pH 5.0, 90°C Pyrococcus furiosus
3.2.1.B28 2.6
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 90°C Pyrococcus furiosus
3.2.1.B28 4.6
-
 4-nitrophenyl beta-D-galactoside pH 5.0, 70°C Pyrococcus furiosus
3.2.1.B28 7.2
-
 4-nitrophenyl beta-D-galactoside pH 5.0, 50°C Pyrococcus furiosus
3.2.1.B28 9.1
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 70°C Pyrococcus furiosus
3.2.1.B28 20
-
 4-nitrophenyl beta-D-glucoside pH 5.0, 50°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 4-nitrophenyl beta-D-galactoside + H2O the enzyme shows constant donor selectivity (Vmax ratio of the 4-nitrophenyl-beta-D-glucoside and 4-nitrophenyl-beta-D-galactoside) as the temperature increased Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O the enzyme shows constant donor selectivity (Vmax ratio of the 4-nitrophenyl-beta-D-glucoside and 4-nitrophenyl-beta-D-galactoside) as the temperature increases Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-galactoside + H2O at 80°C 61% of the activity with 4-nitrophenyl beta-D-glucoside Pyrococcus furiosus 4-nitrophenol + D-galactose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-glucoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 additional information
-
 CelB shows almost constant selcetivity for the glycon part of a donor substrate as the temperature increases. The specificity constant (Vmax/Km) ratio of phenyl-beta-glucoside and phenyl-beta-galactoside substrate decreases only slightly from 2.6 to 2.1 between 50 and 90°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 6.5
-
-
 Saccharolobus solfataricus